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PMID: 3278984 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Inhibitory monoclonal antibody against a (myristylated) small-molecular-weight antigen from Plasmodium falciparum associated with the parasitophorous vacuole membrane.

Infection and immunity ·Vol. 56 ·No. 4 ·1988-04-00 ·Pages 903-9

Kara UA, Stenzel DJ, Ingram LT, Bushell GR, Lopez JA, Kidson C

Abstract

A small-molecular-weight antigen that occurs in asexual blood stages in synchronized cultures of Plasmodium falciparum was detected by a monoclonal antibody which inhibits parasite growth in vitro. This antigen, QF116, showed a molecular weight of 15,000 in parasite strain FCR-3K+ from The Gambia and 19,000 in strain FCQ-27 from Papua New Guinea. The protein did not show significant glycosylation by galactose or glucosamine labeling but was found to be acylated by myristic acid. By using immunogold labeling and electron microscopy, the location of the antigen could be attributed to the parasitophorous vacuole membrane and to inclusions and vesicles residing within the cytoplasm of the erythrocyte host cell.

MeSH Terms
Animals Antibodies, Monoclonal/immunology Antibodies, Protozoan/immunology Antigens, Protozoan/immunology Antigens, Surface/immunology Erythrocytes/parasitology Fluorescent Antibody Technique Immunohistochemistry Immunosorbent Techniques Membrane Proteins/immunology Myristic Acid Myristic Acids/metabolism Neutralization Tests Plasmodium falciparum/growth & development,immunology Vacuoles/immunology
Chemicals
Antibodies, Monoclonal Antibodies, Protozoan Antigens, Protozoan Antigens, Surface Membrane Proteins Myristic Acids Myristic Acid
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Kara U A
Queensland Institute of Medical Research, Brisbane, Australia.
Stenzel D J
Ingram L T
Bushell G R
Lopez J A
Kidson C
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.56.4.903-909.1988
Published
1988-04-00
Pages
903-9
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC259388
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.56.4.903-909.1988 PMC Full Text View journal details
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