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PMID: 3279152 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Developmentally regulated, phospholipase C-mediated release of the major surface glycoprotein of amastigotes of Trypanosoma cruzi.

The Journal of experimental medicine ·Vol. 167 ·No. 2 ·1988-02-01 ·Pages 300-14

Andrews NW, Robbins ES, Ley V, Hong KS, Nussenzweig V

Abstract

The surface of amastigotes of Trypanosoma cruzi is covered by Ssp-4, a major stage-specific glycoprotein. Ssp-4 is anchored to the cell membrane by GPI. It can be metabolically labeled with [3H]myristic acid, and is converted into a hydrophilic form by treatment with the glycan-specific phospholipase C of T. brucei, or after lysis of the parasites in non-ionic detergents. The hydrophilic form of Ssp-4 is recognized by antibodies to the cross-reactive determinant of the variant surface glycoprotein of African trypanosomes. Ssp-4 is progressively shed during the intra- or extracellular development of amastigotes preceding their transformation into epi- and trypomastigotes. We show here that T. cruzi contains a phospholipase C and that most shed Ssp-4 is hydrophilic, does not contain myristic acid, and reacts with anti-CRD. These observations provide strong evidence that phospholipase C mediates the release of this glycosyl-phosphatidylinositol-anchored protein under physiological conditions, as the parasite undergoes differentiation.

MeSH Terms
Animals Antigens, Surface/isolation & purification Cell Differentiation Cell Membrane/metabolism Glycosylation Molecular Weight Phosphatidylinositols/physiology Trypanosoma cruzi/enzymology,growth & development,metabolism Type C Phospholipases/physiology Variant Surface Glycoproteins, Trypanosoma/isolation & purification,metabolism
Chemicals
Antigens, Surface Phosphatidylinositols Variant Surface Glycoproteins, Trypanosoma Type C Phospholipases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Andrews N W
Department of Pathology, New York University Medical Center 10016.
Robbins E S
Ley V
Hong K S
Nussenzweig V
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.167.2.300
Published
1988-02-01
Pages
300-14
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2188833
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.167.2.300 PMC Full Text View journal details
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