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PMID: 3279392 Published · ppublish English Journal Article

The protein factor which binds to the upstream activating sequence of Saccharomyces cerevisiae ENO1 gene.

Nucleic acids research ·Vol. 16 ·No. 4 ·1988-02-25 ·Pages 1407-22

Machida M, Uemura H, Jigami Y, Tanaka H

Abstract

Using a gel retardation assay it was shown that the 87 bp DNA fragment (UAS87) containing the upstream activating sequence (UAS) of S. cerevisiae EN01 gene and a nuclear extract gave rise to three migration-retarded species specific to UAS87. Heat- or proteinase-treatment of the nuclear extract revealed that these species were protein-DNA complexes. The precise binding region of the protein identified by DNaseI protection analysis was found to include a CCAAACA sequence which forms a dyad-symmetrical structure. The amount of one of the three migration-retarded species significantly increased when cells were grown in medium containing a gluconeogenic carbon source. The introduction of pGCR8, a multicopy plasmid containing GCR1 gene, a regulatory gene controlling the expression of several glycolytic enzymes, showed no effect on the amount of three migration-retarded species.

MeSH Terms
Base Sequence Gene Expression Regulation Genes Genes, Fungal Molecular Sequence Data Nuclear Proteins/metabolism Phosphopyruvate Hydratase/genetics Plasmids Protein Binding Saccharomyces cerevisiae/enzymology,genetics
Chemicals
Nuclear Proteins Phosphopyruvate Hydratase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Machida M
National Chemical Laboratory for Industry, Kagaku Gijutsu Kenkyusho, Tsukuba Research Center, Ibaraki, Japan.
Uemura H
Jigami Y
Tanaka H
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/16.4.1407
Published
1988-02-25
Pages
1407-22
Language
English
Region
England
NLM ID
0411011
PMCID
PMC336324
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/16.4.1407 PMC Full Text View journal details
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