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PMID: 3281163 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The yeast SNF3 gene encodes a glucose transporter homologous to the mammalian protein.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 7 ·1988-04-00 ·Pages 2130-4

Celenza JL, Marshall-Carlson L, Carlson M

Abstract

The SNF3 gene is required for high-affinity glucose transport in the yeast Saccharomyces cerevisiae and has also been implicated in control of gene expression by glucose repression. We report here the nucleotide sequence of the cloned SNF3 gene. The predicted amino acid sequence shows that SNF3 encodes a 97-kilodalton protein that is homologous to mammalian glucose transporters and has 12 putative membrane-spanning regions. We also show that a functional SNF3-lacZ gene-fusion product cofractionates with membrane proteins and is localized to the cell surface, as judged by indirect immunofluorescence microscopy. Expression of the fusion protein is regulated by glucose repression.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Fungal Proteins/genetics Gene Expression Regulation/drug effects Genes Genes, Fungal Glucose/pharmacology Humans Molecular Sequence Data Monosaccharide Transport Proteins/genetics Rats/genetics Recombinant Fusion Proteins/genetics Saccharomyces cerevisiae/genetics Sequence Homology, Nucleic Acid Species Specificity
Chemicals
Fungal Proteins Monosaccharide Transport Proteins Recombinant Fusion Proteins Glucose
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Celenza J L
Department of Genetics, Columbia University College of Physicians and Surgeons, New York 10032.
Marshall-Carlson L
Carlson M
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29 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.7.2130
Published
1988-04-00
Pages
2130-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC279942
Subset
IM
Grants
NIGMS NIH HHS · GM34095 · United States
Databases
GENBANK
J03246
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1073/pnas.85.7.2130 PMC Full Text View journal details
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