70K heat shock related proteins stimulate protein translocation into microsomes.

Nature ·Vol. 332 ·No. 6167 ·1988-04-28 ·Pages 805-10

Chirico WJ, Waters MG, Blobel G

Abstract

A yeast cytosol is shown to contain two distinct activities that stimulate protein translocation across microsomal membranes. One activity was purified. It consists of two constitutively expressed 70K heat shock related proteins that increase the rate of translocation. Possible mechanisms of action of these proteins are discussed.

MeSH Terms

Adenosine Triphosphate/metabolism Affinity Labels/metabolism Biological Transport, Active/drug effects Dithiothreitol/pharmacology Electrophoresis, Polyacrylamide Gel Ethylmaleimide/pharmacology Heat-Shock Proteins/pharmacology Intracellular Membranes/drug effects Microsomes/drug effects,metabolism Photochemistry Protein Denaturation Proteins/metabolism Saccharomyces cerevisiae/genetics,metabolism,ultrastructure Urea

Chemicals

Affinity Labels Heat-Shock Proteins Proteins Adenosine Triphosphate Urea Ethylmaleimide Dithiothreitol

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Chirico W J

Laboratory of Cell Biology, Howard Hughes Medical Institute, Rockefeller University, New York, New York 10021.

Waters M G

Blobel G

Article Info

Journal

Nature

Abbr.

Nature

ISSN

0028-0836

DOI

10.1038/332805a0

Published

1988-04-28

Pages

805-10

Language

English

Region

England

NLM ID

0410462

Subset

External Links

PubMed source DOI: 10.1038/332805a0 View journal details

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