A colony procedure for transformation of Saccharomyces cerevisiae.

Current genetics ·Vol. 13 ·No. 1 ·1988-00-00 ·Pages 21-3

Keszenman-Pereyra D, Hieda K

Abstract

A rapid and simple yeast transformation procedure has been developed using colonies on agar plates. Saccharomyces cerevisiae SHY3 cells were picked up from colonies on YPD plates grown freshly or stored at 4 degrees C and incubated with M13RK9-T DNA at 30 degrees C for 1-2 h in a solution of Li+, Ca2+, Mg2+, triacetin and polyethylene glycol. About 3,500 transformants were obtained per microgram of double stranded M13RK9-T DNA. Unlike the existing spheroplast techniques, single stranded M13RK9-T DNA transformed intact cells below one-hundredth frequency of the duplex form.

MeSH Terms

Genetic Engineering/methods Saccharomyces cerevisiae/genetics,growth & development Transformation, Genetic

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Keszenman-Pereyra D

Department of Physics, Rikkyo University, Tokyo, Japan.

Hieda K

References (17)

17 references, click to expand

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Article Info

Journal

Current genetics

Abbr.

Curr Genet

ISSN

0172-8083

DOI

10.1007/BF00365751

Published

1988-00-00

Pages

21-3

Language

English

Region

United States

NLM ID

8004904

Subset

External Links

PubMed source DOI: 10.1007/BF00365751 View journal details

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A colony procedure for transformation of Saccharomyces cerevisiae.

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