Evaluation of a nonisotopically labeled oligonucleotide probe to detect the heat-stable enterotoxin gene of Escherichia coli by the DNA colony hybridization test.

Journal of clinical microbiology ·Vol. 26 ·No. 4 ·1988-04-00 ·Pages 784-6

Nishibuchi M, Arita M, Honda T, Miwatani T

Abstract

A commercially available, alkaline-phosphatase-conjugated oligonucleotide probe for detecting the heat-stable enterotoxin gene of Escherichia coli was compared with cloned gene probes by examining E. coli isolates from traveler's diarrhea by DNA colony hybridization tests. The oligonucleotide probe was useful in specifically identifying the so-called STh gene. No deproteinization of sample was necessary to prepare the colony blots.

MeSH Terms

Animals Bacterial Toxins/genetics DNA, Bacterial/genetics Enterotoxins/genetics Escherichia coli/genetics,pathogenicity Escherichia coli Proteins Genes, Bacterial Humans Mice Nucleic Acid Hybridization Oligodeoxyribonucleotides/genetics Predictive Value of Tests

Chemicals

Bacterial Toxins DNA, Bacterial Enterotoxins Escherichia coli Proteins Oligodeoxyribonucleotides heat stable toxin (E coli)

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Nishibuchi M

Research Institute for Microbial Diseases, Osaka University, Japan.

Arita M

Honda T

Miwatani T

References (25)

25 references, click to expand

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Article Info

Journal

Journal of clinical microbiology

Abbr.

J Clin Microbiol

ISSN

0095-1137

DOI

10.1128/jcm.26.4.784-786.1988

Published

1988-04-00

Pages

784-6

Language

English

Region

United States

NLM ID

7505564

PMCID

PMC266450

Subset

External Links

PubMed source DOI: 10.1128/jcm.26.4.784-786.1988 PMC Full Text View journal details

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Evaluation of a nonisotopically labeled oligonucleotide probe to detect the heat-stable enterotoxin gene of Escherichia coli by the DNA colony hybridization test.

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