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PMID: 3288970 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Bacteriophage T7 late promoters with point mutations: quantitative footprinting and in vivo expression.

Nucleic acids research ·Vol. 16 ·No. 10 ·1988-05-25 ·Pages 4511-24

Chapman KA, Gunderson SI, Anello M, Wells RD, Burgess RR

Abstract

暂无摘要

MeSH Terms
Base Sequence DNA-Directed DNA Polymerase/metabolism Escherichia coli/enzymology,genetics Genes, Viral Molecular Sequence Data Mutation Promoter Regions, Genetic T-Phages/enzymology,genetics Templates, Genetic Transcription, Genetic
Chemicals
DNA-Directed DNA Polymerase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Chapman K A
McArdle Laboratory for Cancer Research, University of Wisconsin, Madison 53706.
Gunderson S I
Anello M
Wells R D
Burgess R R
References (15)
15 references, click to expand
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    Nature. 1979 Jul 5;280(5717):35-9 PMID: 15305578
  2. A simple method of preparing large amounts of phiX174 RF 1 supercoiled DNA.
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  3. Studies on T3-induced ribonucleic acid polymerase. 3. Purification and characterization of the T3-induced ribonucleic acid polymerase from bacteriophage T3-infected Escherichia coli cells.
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  4. Coumerimycin A1: A preferential inhibitor of replicative DNA synthesis in Escherichia coli. II. In vivo characterization.
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  5. Four T7 RNA polymerase promoters contain an identical 23 bp sequence.
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  6. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  7. Promoter melting by T7 ribonucleic acid polymerase as detected by single-stranded endonuclease digestion.
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  8. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  9. T7 ribonucleic acid polymerase-promotor interactions.
    Biochemistry. 1981 Aug 18;20(17):4884-92 PMID: 6271174
  10. Complete nucleotide sequence of bacteriophage T7 DNA and the locations of T7 genetic elements.
    J Mol Biol. 1983 Jun 5;166(4):477-535 PMID: 6864790
  11. Effects of the mutant sigma allele rpoD800 on the synthesis of specific macromolecular components of the Escherichia coli K12 cell.
    J Mol Biol. 1984 Jan 25;172(3):283-300 PMID: 6198525
  12. Cloning and expression of the gene for bacteriophage T7 RNA polymerase.
    Proc Natl Acad Sci U S A. 1984 Apr;81(7):2035-9 PMID: 6371808
  13. A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes.
    Proc Natl Acad Sci U S A. 1985 Feb;82(4):1074-8 PMID: 3156376
  14. Interactions of the RNA polymerase of bacteriophage T7 with its promoter during binding and initiation of transcription.
    Proc Natl Acad Sci U S A. 1986 Jun;83(11):3614-8 PMID: 3459146
  15. Construction of bacteriophage T7 late promoters with point mutations and characterization by in vitro transcription properties.
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
Published
1988-05-25
Pages
4511-24
Language
English
Region
England
NLM ID
0411011
PMCID
PMC336645
Subset
IM
Grants
NCI NIH HHS · CA 07175 · United States
NCI NIH HHS · CA 23076 · United States
NIGMS NIH HHS · GM 30822 · United States
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