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PMID: 3290899 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

FLP recombinase is an enzyme.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 13 ·1988-07-00 ·Pages 4628-32

Gates CA, Cox MM

Abstract

The FLP protein of the yeast 2-microns plasmid catalyzes intermolecular site-specific recombination with a turnover number of approximately equal to 0.12 min-1 (per FLP monomer) for relaxed DNA substrates. Under conditions that enhance its stability, the protein can be used in catalytic rather than stoichiometric amounts. The reaction rate exhibits a strong dependence on FLP protein concentration even when the protein is present in excess relative to available recombination sites.

MeSH Terms
Base Sequence DNA Nucleotidyltransferases/metabolism DNA, Fungal/metabolism Fungal Proteins/metabolism Plasmids Recombination, Genetic Saccharomyces cerevisiae/enzymology
Chemicals
DNA, Fungal Fungal Proteins DNA Nucleotidyltransferases FLP recombinase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Gates C A
Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin-Madison 53706.
Cox M M
References (30)
30 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.13.4628
Published
1988-07-00
Pages
4628-32
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC280488
Subset
IM
Grants
NIAID NIH HHS · AI00599 · United States
NIGMS NIH HHS · GM37835 · United States
External Links
PubMed source DOI: 10.1073/pnas.85.13.4628 PMC Full Text View journal details
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