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PMID: 3291857 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Proteus mirabilis urease. Partial purification and inhibition by boric acid and boronic acids.

The Biochemical journal ·Vol. 250 ·No. 3 ·1988-03-15 ·Pages 917-20

Breitenbach JM, Hausinger RP

Abstract

Urease was purified 800-fold and partially characterized from Proteus mirabilis, the predominant microorganism associated with urinary stones. Boric acid is a rapid reversible competitive inhibitor of urease. The pH-dependence of inhibition exhibited pKa values of 6.25 and 9.3, where the latter value is probably due to the inherent pKa of boric acid. Three boronic acids also were shown to inhibit urease competitively.

MeSH Terms
Boric Acids/pharmacology Boronic Acids/pharmacology Chromatography, Liquid Hydrogen-Ion Concentration Kinetics Proteus mirabilis/enzymology Urease/antagonists & inhibitors,isolation & purification
Chemicals
Boric Acids Boronic Acids Urease boric acid
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Breitenbach J M
Department of Microbiology and Public Health, Michigan State University, East Lansing 48824-1101.
Hausinger R P
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2500917
Published
1988-03-15
Pages
917-20
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1148943
Subset
IM
Grants
NCRR NIH HHS · 2-507 RRO 7049-15 · United States
NIAID NIH HHS · AI 22387 · United States
External Links
PubMed source DOI: 10.1042/bj2500917 PMC Full Text View journal details
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