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PMID: 3294333 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Human sperm carbohydrate antigens defined by an antisperm human monoclonal antibody derived from an infertile woman bearing antisperm antibodies in her serum.

The Journal of experimental medicine ·Vol. 168 ·No. 1 ·1988-07-01 ·Pages 343-56

Tsuji Y, Clausen H, Nudelman E, Kaizu T, Hakomori S, Isojima S

Abstract

The epitope structure of the human sperm antigen reacting with antibodies present in sera of infertile women has been studied using mAb H6-3C4, which produces immobilization of human sperm in the presence of complement. Another antibody, NUH2, which also induces human sperm immobilization, was used to substantiate the presence of a receptor on sperm involved in susceptibility to immobilization. Both antibodies defined type 2 chain polylactosamine structure. H6-3C4 is directed to internally located repetitive N-acetyllactosamine, i.e., sialyl-i, i, or fucosyl-i. NUH2 defines binary alpha 2----3 sialyl type 2 chain, i.e., sialyl-I. Thus, the presence of antibodies in the sera of infertile women directed to sperm lactosaminoglycan or lactosaminolipid could be the basis for infertility in these cases.

MeSH Terms
Amino Sugars/immunology Antibodies, Monoclonal/immunology Antibody Specificity Antigens/immunology Carbohydrate Sequence Carbohydrates/immunology Chromatography, Thin Layer Complement System Proteins/immunology Female Fluorescent Antibody Technique Hemagglutination Humans Immunoassay Infertility, Female/immunology Male Molecular Sequence Data Radioimmunoassay Sperm Motility Spermatozoa/immunology
Chemicals
Amino Sugars Antibodies, Monoclonal Antigens Carbohydrates N-acetyllactosamine Complement System Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Tsuji Y
Biomembrane Institute, University of Washington, Seattle 98119.
Clausen H
Nudelman E
Kaizu T
Hakomori S
Isojima S
References (35)
35 references, click to expand
  1. The hapten structure of a developmentally regulated glycolipid antigen (SSEA-1) isolated from human erythrocytes and adenocarcinoma: a preliminary note.
    Biochem Biophys Res Commun. 1981 Jun;100(4):1578-86 PMID: 6117281
  2. Stage-specific embryonic antigens (SSEA-3 and -4) are epitopes of a unique globo-series ganglioside isolated from human teratocarcinoma cells.
    EMBO J. 1983;2(12):2355-61 PMID: 6141938
  3. A ceramide tetrasaccharide of human erythrocyte membrane reacting with anti-type XIV pneumococcal polysaccharide antiserum.
    Biochim Biophys Acta. 1973 Dec 13;330(2):147-55 PMID: 4149633
  4. Blood group ABH and Ii antigens of human erythrocytes: chemistry, polymorphism, and their developmental change.
    Semin Hematol. 1981 Jan;18(1):39-62 PMID: 6782678
  5. Gangliosides and other glycolipids of human placenta.
    Acta Chem Scand. 1965;19(6):1506-7 PMID: 5850147
  6. A series of human erythrocyte glycosphingolipids reacting to the monoclonal antibody directed to a developmentally regulated antigen SSEA-1.
    J Biol Chem. 1982 Dec 25;257(24):14865-74 PMID: 6129244
  7. Human placental (fetal) fibronectin: increased glycosylation and higher protease resistance than plasma fibronectin. Presence of polylactosamine glycopeptides and properties of a 44-kilodalton chymotryptic collagen-binding domain: difference from human plasma fibronectin.
    J Biol Chem. 1984 Mar 25;259(6):3962-70 PMID: 6423638
  8. Infertility in female guinea pigs induced by injection of homologous sperm.
    Am J Obstet Gynecol. 1959 Aug;78(2):276-8 PMID: 13670195
  9. Cold agglutinins--antibodies with biological diversity.
    Clin Immunol Rev. 1984;3(1):131-68 PMID: 6207971
  10. A human monoclonal antibody directed to blood group i antigen: heterohybridoma between human lymphocytes from regional lymph nodes of a lung cancer patient and mouse myeloma.
    J Immunol. 1986 Jun 1;136(11):4163-8 PMID: 2422274
  11. Concepts and experiments in immunoreproduction.
    Fertil Steril. 1967 Mar-Apr;18(2):153-66 PMID: 5336043
  12. New globoseries glycosphingolipids in human teratocarcinoma reactive with the monoclonal antibody directed to a developmentally regulated antigen, stage-specific embryonic antigen 3.
    J Biol Chem. 1983 Jul 25;258(14):8934-42 PMID: 6863318
  13. Further studies on sperm-immobilizing antibody found in sera of unexplained cases of sterility in women.
    Am J Obstet Gynecol. 1972 Jan 15;112(2):199-207 PMID: 5061795
  14. ANTISPERMATOZOAL ANTIBODY AND UNEXPLAINED INFERTILITY.
    Am J Obstet Gynecol. 1964 May 1;89:6-9 PMID: 14155015
  15. A monoclonal antibody, EC-1, derived from a syngeneically multiparous mouse alters in vitro fertilization and development.
    J Reprod Immunol. 1985 Aug;8(1):45-59 PMID: 2413203
  16. New gangliosides from human erythrocytes.
    J Biol Chem. 1983 Nov 25;258(22):13857-66 PMID: 6643456
  17. Shared antigenic determinant expressed on various mammalian melanoma cells.
    Gan. 1984 May;75(5):418-26 PMID: 6204902
  18. Detection of gangliosides that bind cholera toxin: direct binding of 125I-labeled toxin to thin-layer chromatograms.
    Anal Biochem. 1980 Dec;109(2):399-402 PMID: 7224165
  19. Blood group i and I activities of "lacto-N-norhexaosylceramide" and its analogues: the structural requirements for i-specificities.
    Biochem Biophys Res Commun. 1978 Apr 28;81(4):1286-93 PMID: 78708
  20. Factors affecting expression of glycolipid tumor antigens: influence of ceramide composition and coexisting glycolipid on the antigenicity of gangliotriaosylceramide in murine lymphoma cells.
    Cancer Res. 1983 Oct;43(10):4997-5005 PMID: 6860431
  21. Characterization of a blood group I-active ganglioside. Structural requirements for I and i specificities.
    J Biol Chem. 1979 May 10;254(9):3221-8 PMID: 107170
  22. The incidence of sperm-agglutinating antibodies in the serum of infertile women.
    Fertil Steril. 1969 Mar-Apr;20(2):275-8 PMID: 5767273
  23. Stage-specific expression of three cell surface carbohydrate antigens during murine spermatogenesis detected with monoclonal antibodies.
    Dev Biol. 1984 May;103(1):117-28 PMID: 6370756
  24. Changes in the expression and polarization of blood group I and i antigens in post-implantation embryos and teratocarcinomas of mouse associated with cell differentiation.
    Exp Cell Res. 1981 Jan;131(1):185-95 PMID: 7004456
  25. Glycolipid antigens with blood group I and i specificities from human adult and umbilical cord erythrocytes.
    J Immunol. 1984 Aug;133(2):835-42 PMID: 6203982
  26. Anti-i: a new cold antibody.
    Nature. 1960 Nov 26;188:753 PMID: 13767180
  27. A monoclonal antibody defining a binary N-acetyllactosaminyl structure in lactoisooctaosylceramide (IV6Gal beta 1----4GlcNAcnLc6): a useful probe for determining differential glycosylation patterns between normal and transformed human fibroblasts.
    Mol Immunol. 1986 Jul;23(7):747-54 PMID: 3796623
  28. Isolation and characterization of gangliosides with a new sialosyl linkage and core structures. II. Gangliosides of human erythrocyte membranes.
    J Biol Chem. 1979 Sep 10;254(17):8223-9 PMID: 468822
  29. Two monoclonal anticarbohydrate antibodies directed to glycosphingolipids with a lacto-N-glycosyl type II chain.
    J Biol Chem. 1981 Nov 10;256(21):10967-72 PMID: 7287743
  30. Monosialogangliosides of rabbit skeletal muscle. Characterization of N-acetylneuraminosyl lacto-N-noroctaosyl ceramide.
    J Biochem. 1981 Apr;89(4):1253-64 PMID: 7251579
  31. A monoclonal antibody directed to N-acetylneuraminosyl-alpha 2 leads to 6-galactosyl residue in gangliosides and glycoproteins.
    J Biol Chem. 1983 Oct 10;258(19):11819-22 PMID: 6194157
  32. Developmental change and genetic defect in the carbohydrate structure of band 3 glycoprotein of human erythrocyte membrane.
    J Biol Chem. 1979 May 25;254(10):3700-3 PMID: 438154
  33. Density-dependent recognition of cell surface GM3 by a certain anti-melanoma antibody, and GM3 lactone as a possible immunogen: requirements for tumor-associated antigen and immunogen.
    J Immunol. 1987 Nov 1;139(9):3171-6 PMID: 3668254
  34. Release of oligosaccharides from various glycosphingolipids by endo-beta-galactosidase.
    J Biol Chem. 1978 Oct 10;253(19):6814-9 PMID: 99449
  35. Establishment and characterization of a human hybridoma secreting monoclonal antibody with high titers of sperm immobilizing and agglutinating activities against human seminal plasma.
    J Reprod Immunol. 1987 Jan;10(1):67-78 PMID: 3295232
Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.168.1.343
Published
1988-07-01
Pages
343-56
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2188971
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.168.1.343 PMC Full Text View journal details
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