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PMID: 3294789 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification and expression of a copy number control gene in the IncFIII hemolytic plasmid pSU316.

Journal of bacteriology ·Vol. 169 ·No. 6 ·1987-06-00 ·Pages 2405-9

Andrés I, Rodriguez JC, Barbadillo J, Ortiz JM

Abstract

A DNA fragment carrying both the IncFIII determinant and a copy number control gene of the hemolytic plasmid pSU316 has been cloned in pBR322. Deletion derivatives of the hybrid plasmid generated by Bal 31 digestion, which no longer exhibit the IncFIII phenotype, fall into two complementation groups when tested against a pSU316 miniplasmid derivative. Type 1 mutants exhibit the copy number control (Cop+) phenotype whereas type 2 mutants do not. Restriction analysis of type 1 and type 2 mutants allowed us to locate the cop gene of pSU316 in a 700-base-pair fragment adjacent to the IncFIII determinant. Plasmid expression in a minicell system suggests that the product of the cop gene of pSU316 could be a 13,000-dalton protein.

MeSH Terms
Bacterial Proteins/genetics Cloning, Molecular DNA Replication Escherichia coli/genetics Gene Expression Regulation Genes, Bacterial Genetic Complementation Test Molecular Weight Plasmids
Chemicals
Bacterial Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Andrés I
Rodriguez J C
Barbadillo J
Ortiz J M
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21 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.169.6.2405-2409.1987
Published
1987-06-00
Pages
2405-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC212077
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.169.6.2405-2409.1987 PMC Full Text View journal details
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