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PMID: 3295881 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Expression of the alpha-bungarotoxin binding site of the nicotinic acetylcholine receptor by Escherichia coli transformants.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 12 ·1987-06-00 ·Pages 4318-21

Gershoni JM

Abstract

Restriction fragments of DNA derived from a cDNA clone of the alpha subunit of the acetylcholine receptor were subcloned in Escherichia coli by using the trpE fusion vector, pATH2. Transformants expressing the amino acid sequences 166-315 or 166-200 are shown to produce a chimeric protein that bound alpha-bungarotoxin. Moreover, it is shown that sufficient amounts of toxin-binding proteins can be generated by individual colonies of bacteria. This provides a new approach for gene selection via functional expression--i.e., ligand overlays of colony blots.

MeSH Terms
Animals Bungarotoxins/metabolism Cloning, Molecular DNA/metabolism Escherichia coli/genetics Genes Genetic Vectors Plasmids Receptors, Cholinergic/genetics Receptors, Nicotinic/genetics Torpedo alpha7 Nicotinic Acetylcholine Receptor
Chemicals
Bungarotoxins Receptors, Cholinergic Receptors, Nicotinic alpha7 Nicotinic Acetylcholine Receptor DNA
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Gershoni J M
References (18)
18 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.12.4318
Published
1987-06-00
Pages
4318-21
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC305076
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.84.12.4318 PMC Full Text View journal details
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