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PMID: 3298998 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Promoter switching during development and the termination site of the sigma 43 operon of Bacillus subtilis.

Molecular & general genetics : MGG ·Vol. 207 ·No. 1 ·1987-04-00 ·Pages 114-9

Wang LF, Doi RH

Abstract

Sequencing data indicated that the RNA polymerase sigma 43 operon of Bacillus subtilis consisted of three genes, P23 (function unknown), dnaE (DNA primase), and rpoD (sigma 43) (Wang and Doi 1986a). S1 nuclease mapping experiments with RNA from various stages of growth demonstrated the presence of two overlapping sigma 43 promoters that controlled the expression of the operon during growth and a sigma 37 promoter that regulated the expression of the operon during the sporulation phase. This promoter switching mechanism ensured that this important operon would be expressed during different nutritional states of the cell and also illustrated a function for the minor RNA polymerase sigma 37 holoenzyme in the expression of genes which are normally expressed during the logarithmic phase of growth. The location of the transcription termination signal confirmed that the sigma 43 operon consists of three genes.

MeSH Terms
Bacterial Proteins/genetics,metabolism DNA-Directed RNA Polymerases/metabolism Escherichia coli/genetics,physiology Gene Expression Regulation Genes Genes, Bacterial Genes, Regulator Operon Promoter Regions, Genetic Sigma Factor/genetics Spores, Bacterial Terminator Regions, Genetic Transcription Factors/genetics
Chemicals
Bacterial Proteins Sigma Factor Transcription Factors DNA-Directed RNA Polymerases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wang L F
Doi R H
References (28)
28 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00331498
Published
1987-04-00
Pages
114-9
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NIGMS NIH HHS · GM 19673-14 · United States
Databases
GENBANK
X05334
External Links
PubMed source DOI: 10.1007/BF00331498
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