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PMID: 3299003 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Catalase has only a minor role in protection against near-ultraviolet radiation damage in bacteria.

Molecular & general genetics : MGG ·Vol. 207 ·No. 1 ·1987-04-00 ·Pages 68-72

Eisenstark A, Perrot G

Abstract

In bacterial cells near-ultraviolet radiation (NUV) generates H2O2 which can be decomposed by endogenous catalase to H2O and O2. To assess the roles of H2O2 and catalase in NUV lethality, we manipulated the amount of intracellular catalase (a) by the use of mutant and plasmid strains with altered endogenous catalase, (b) physiologically, by the addition of glucose, and (c) by induction of catalase synthesis with oxidizing agents. Not only was there no direct correlation between NUV-resistance and catalase activity, but in some cases the correlation was inverse. Also, while there was correlation between NUV and H2O2 sensitivity for most strains tested, there were a number of exceptions which indicates that the modes of killing were different for the two agents.

MeSH Terms
Bacterial Proteins/genetics,physiology Catalase/genetics,physiology Escherichia coli/enzymology,genetics,radiation effects Glucose/pharmacology Hydrogen Peroxide/metabolism Radiation Tolerance/drug effects Salmonella typhimurium/enzymology,genetics,radiation effects Ultraviolet Rays
Chemicals
Bacterial Proteins Hydrogen Peroxide Catalase Glucose
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Eisenstark A
Perrot G
References (26)
26 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00331492
Published
1987-04-00
Pages
68-72
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
PHS HHS · RFR 07053 · United States
External Links
PubMed source DOI: 10.1007/BF00331492
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