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PMID: 3299263 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The yeast DNA polymerase I transcript is regulated in both the mitotic cell cycle and in meiosis and is also induced after DNA damage.

Nucleic acids research ·Vol. 15 ·No. 13 ·1987-07-10 ·Pages 5017-30

Johnston LH, White JH, Johnson AL, Lucchini G, Plevani P

Abstract

Using mitotic cultures synchronised by a feed-starve protocol or by elutriation, we have shown that the yeast DNA polymerase I gene is periodically expressed with its transcript increasing at least 100-fold in late G1 with a peak around the G1/S phase boundary. This is precisely the same interval of the cell cycle in which three other yeast DNA synthesis genes, CDC8, CDC9 and CDC21, have been found to be periodically expressed (White et al 1987. Expl. Cell. Res., in press). The polymerase I transcript is also regulated in meiosis, showing an overall fluctuation in level of some 20-fold, with a peak at about mid-S phase. In addition, following irradiation with 50J/m2 ultraviolet light, there was a 20-fold increase in the transcript, starting after 30 minutes and reaching a peak two hours later. These results indicate that DNA polymerase I is subject to a complex control and imply that it has a role in both DNA synthesis and DNA repair.

MeSH Terms
Cell Cycle DNA Damage DNA Polymerase I/genetics Gene Expression Regulation Genes Genes, Fungal Meiosis Mitosis Nucleic Acid Hybridization Saccharomyces cerevisiae/cytology,enzymology,genetics Transcription, Genetic
Chemicals
DNA Polymerase I
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Johnston L H
White J H
Johnson A L
Lucchini G
Plevani P
References (24)
24 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.13.5017
Published
1987-07-10
Pages
5017-30
Language
English
Region
England
NLM ID
0411011
PMCID
PMC305944
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/15.13.5017 PMC Full Text View journal details
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