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PMID: 3299269 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

High frequencies of short frameshifts in poly-CA/TG tandem repeats borne by bacteriophage M13 in Escherichia coli K-12.

Nucleic acids research ·Vol. 15 ·No. 13 ·1987-07-10 ·Pages 5323-38

Levinson G, Gutman GA

Abstract

Slipped-strand mispairing (SSM) may play an major role in repetitive DNA sequence evolution by generating large numbers of short frameshift mutations within simple tandem repeats. Here we examine the frequency and size spectrum of frameshifts generated within poly-CA/TG sequences inserted into bacteriophage M13 in Escherichia coli hosts. The frequency of detectable frameshifts within a 40 bp tract of poly-CA/TG is greater than one percent and increases more than linearly with length, being lower by a factor of four in a 22 bp target sequence. The frequency increases more than 13-fold in mutL and mutS host cells, suggesting that a high proportion of frameshift events are normally repaired by methyl-directed mismatch repair. Of the 87 sequenced frameshifts in this study, 96% result from deletion or insertion of only or two 2 bp repeat units. The most frequent events are 2 bp deletions, 2 bp insertions, and 4 bp deletions, the relative frequencies of these events being about 18:6:1.

MeSH Terms
Base Composition Base Sequence Biological Evolution Chromosome Deletion Coliphages/genetics DNA Repair Escherichia coli/genetics Mutation Polynucleotides/genetics Repetitive Sequences, Nucleic Acid
Chemicals
Polynucleotides
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Levinson G
Gutman G A
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.13.5323
Published
1987-07-10
Pages
5323-38
Language
English
Region
England
NLM ID
0411011
PMCID
PMC305964
Subset
IM
Grants
NIAID NIH HHS · AI-14774 · United States
NIAID NIH HHS · AI-21366 · United States
NICHD NIH HHS · HD07029 · United States
External Links
PubMed source DOI: 10.1093/nar/15.13.5323 PMC Full Text View journal details
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