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PMID: 3305520 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

A yeast mutant defective at an early stage in import of secretory protein precursors into the endoplasmic reticulum.

The Journal of cell biology ·Vol. 105 ·No. 2 ·1987-08-00 ·Pages 633-45

Deshaies RJ, Schekman R

Abstract

We have devised a genetic selection for mutant yeast cells that fail to translocate secretory protein precursors into the lumen of the endoplasmic reticulum (ER). Mutant cells are selected by a procedure that requires a signal peptide-containing cytoplasmic enzyme chimera to remain in contact with the cytosol. This approach has uncovered a new secretory mutant, sec61, that is thermosensitive for growth and that accumulates multiple secretory and vacuolar precursor proteins that have not acquired any detectable posttranslational modifications associated with translocation into the ER. Preproteins that accumulate at the sec61 block sediment with the particulate fraction, but are exposed to the cytosol as judged by sensitivity to proteinase K. Thus, the sec61 mutation defines a gene that is required for an early cytoplasmic or ER membrane-associated step in protein translocation.

MeSH Terms
Endoplasmic Reticulum/metabolism Genes Genes, Fungal Genotype Mutation Protein Biosynthesis Protein Precursors/genetics Protein Processing, Post-Translational Saccharomyces cerevisiae/genetics,metabolism Transcription, Genetic
Chemicals
Protein Precursors
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Deshaies R J
Schekman R
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58 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.105.2.633
Published
1987-08-00
Pages
633-45
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2114772
Subset
IM
Grants
NIGMS NIH HHS · GM-26755 · United States
External Links
PubMed source DOI: 10.1083/jcb.105.2.633 PMC Full Text View journal details
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