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PMID: 330738 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Two-color immunofluorescence using a fluorescence-activated cell sorter.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society ·Vol. 25 ·No. 7 ·1977-07-00 ·Pages 899-907

Loken MR, Parks DR, Herzenberg LA

Abstract

A technique for the analysis of fluorescein and rhodamine in a flow system using a single wavelength of excitation is described. Both optics and electronics are used to discriminate the fluorescein and rhodamine signals. This technique has been used to study the relationship between immunoglobulin M and immunoglobulin D on mouse splenic lymphocytes.

MeSH Terms
Cell Separation/instrumentation Fluoresceins Fluorescent Antibody Technique/instrumentation Immunoglobulin D/analysis Immunoglobulin M/analysis Lymphocytes/immunology Rhodamines Spleen/cytology
Chemicals
Fluoresceins Immunoglobulin D Immunoglobulin M Rhodamines
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Loken M R
Parks D R
Herzenberg L A
Article Info
Journal
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
Abbr.
J Histochem Cytochem
ISSN
0022-1554
DOI
10.1177/25.7.330738
Published
1977-07-00
Pages
899-907
Language
English
Region
United States
NLM ID
9815334
Subset
IM
External Links
PubMed source DOI: 10.1177/25.7.330738 View journal details
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