Improved sensitivity in assays for binding of novel beta-lactam antibiotics to penicillin-binding proteins of Escherichia coli.

Antimicrobial agents and chemotherapy ·Vol. 31 ·No. 8 ·1987-08-00 ·Pages 1271-3

Bush K, Smith SA, Ohringer S, Tanaka SK, Bonner DP

Abstract

Tigemonam and temocillin, but not aztreonam, bound to penicillin-binding proteins (PBPs) 1a and 3 of Escherichia coli with apparent improved affinity when challenged with benzylpenicillin at lowered temperatures. Half times for deacylation of the tigemonam-PBP complexes were shorter than were those of the corresponding aztreonam-PBP complexes. The implications of the routine testing of PBP affinities are discussed.

MeSH Terms

Anti-Bacterial Agents/metabolism Bacterial Proteins Carrier Proteins/metabolism Cell Membrane/metabolism Dealkylation Escherichia coli/metabolism Hexosyltransferases Kinetics Microbial Sensitivity Tests Monobactams/metabolism Muramoylpentapeptide Carboxypeptidase/metabolism Penicillin G/metabolism Penicillin-Binding Proteins Peptidyl Transferases Protein Binding Temperature

Chemicals

Anti-Bacterial Agents Bacterial Proteins Carrier Proteins Monobactams Penicillin-Binding Proteins tigemonam Peptidyl Transferases Hexosyltransferases Muramoylpentapeptide Carboxypeptidase Penicillin G

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Bush K

Smith S A

Ohringer S

Tanaka S K

Bonner D P

References (12)

12 references, click to expand

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Antimicrob Agents Chemother. 1987 Feb;31(2):226-9 PMID: 3551830
Affinity of temocillin for Escherichia coli K-12 penicillin-binding proteins.
Antimicrob Agents Chemother. 1984 Sep;26(3):335-8 PMID: 6391369
Penicillin-sensitive enzymes in peptidoglycan biosynthesis.
Crit Rev Microbiol. 1985;11(4):299-396 PMID: 3888533
In vitro and in vivo antibacterial activities of carumonam (AMA-1080), a new N-sulfonated monocyclic beta-lactam antibiotic.
Antimicrob Agents Chemother. 1985 May;27(5):821-7 PMID: 3874598
Active-site-serine D-alanyl-D-alanine-cleaving-peptidase-catalysed acyl-transfer reactions. Procedures for studying the penicillin-binding proteins of bacterial plasma membranes.
Biochem J. 1986 Apr 1;235(1):159-65 PMID: 3741376
In vitro evaluation of tigemonam, a novel oral monobactam.
Antimicrob Agents Chemother. 1987 Feb;31(2):219-25 PMID: 3105448
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Article Info

Journal

Antimicrobial agents and chemotherapy

Abbr.

Antimicrob Agents Chemother

ISSN

0066-4804

DOI

10.1128/AAC.31.8.1271

Published

1987-08-00

Pages

1271-3

Language

English

Region

United States

NLM ID

0315061

PMCID

PMC174917

Subset

External Links

PubMed source DOI: 10.1128/AAC.31.8.1271 PMC Full Text View journal details

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Improved sensitivity in assays for binding of novel beta-lactam antibiotics to penicillin-binding proteins of Escherichia coli.

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