Home LiteratureArticle Details
PMID: 330881 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Localization of single-chain interruptions in bacteriophage T5 DNA I. Electron microscopic studies.

Journal of virology ·Vol. 23 ·No. 3 ·1977-09-00 ·Pages 725-36

Scheible PP, Rhoades EA, Rhoades M

Abstract

Bacteriophage T5 DNA was examined in an electron microscope after limited digestion with exonuclease III from Escherichia coli. The effect of the exonuclease treatment was to convert each naturally occurring single-chain interruption in T5 DNA into a short segment of single-stranded DNA. The locations of these segments were determined for T5st(+) DNA, T5st(0) DNA, and fragments of T5st(0) DNA generated by EcoRI restriction endonuclease. The results indicate that single-chain interruptions occurr in a variable, but nonrandom, manner in T5 DNA. T5st(+) DNA has four principal interruptions located at sites approximately 7.9, 18.5, 32.6, and 64.8% from one end of the molecule. Interruptions occur at these sites in 80 to 90% of the population. A large number of additional sites, located primarily at the ends of the DNA, contain interruptions at lower frequencies. The average number of interruptions per genome, as determined by this method, is 8. A similar distribution of breaks occurs in T5st(0) DNA, except that the 32.6% site is missing. At least one of the principal interruptions is reproducibly located within an interval of 0.2% of the entire DNA.

MeSH Terms
Coliphages/analysis DNA Restriction Enzymes/metabolism DNA, Single-Stranded/analysis DNA, Viral/analysis Escherichia coli/enzymology Exonucleases/metabolism Microscopy, Electron Molecular Weight
Chemicals
DNA, Single-Stranded DNA, Viral Exonucleases DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Scheible P P
Rhoades E A
Rhoades M
References (26)
26 references, click to expand
  1. Denaturation mapping and chromosome structure in bacteriophage T5.
    Virology. 1975 Nov;68(1):166-72 PMID: 242116
  2. Localization of single-chain interruptions in bacteriophage T5 DNA. II. Electrophoretic studies.
    J Virol. 1977 Sep;23(3):737-50 PMID: 894794
  3. Cleavage of T5 DNA by the Escherichia coli R-I restriction endonuclease.
    Virology. 1975 Mar;64(1):170-9 PMID: 1090074
  4. Molecular weights of DNA from bacteriophages T5, T5st(O), BF23, and BF23st(4).
    J Virol. 1975 Jan;17(1):296-7 PMID: 1206802
  5. Unique double-stranded fragments of bacteriophage T5 DNA resulting from preferential shear-induced breakage at nicks.
    Proc Natl Acad Sci U S A. 1974 May;71(5):2108-12 PMID: 4525320
  6. Terminal repetition in the DNA of bacteriophage T5.
    J Mol Biol. 1972 Aug 21;69(2):187-200 PMID: 4560946
  7. Cleavage of adenovirus type 2 DNA into six unique fragments by endonuclease R-RI.
    Proc Natl Acad Sci U S A. 1973 Jan;70(1):200-4 PMID: 4567334
  8. Structure and function of the genome of coliphage T5. 1. The physical structure of the chromosome of T5 + .
    Eur J Biochem. 1973 Mar 15;33(3):517-28 PMID: 4571500
  9. Location of the first step transfer fragment and single-strand interruptions in T5stO bacteriophage DNA.
    J Mol Biol. 1973 Apr 5;75(2):213-34 PMID: 4580675
  10. Molecular weights of coliphages and coliphage DNA. IV. Molecular weights of DNA from bacteriophages T4, T5 and T7 and the general problem of determination of M.
    J Mol Biol. 1970 Dec 28;54(3):567-77 PMID: 4923669
  11. Enzymatic removal and replacement of nucleotides at single strand breaks in deoxyribonucleic acid.
    J Biol Chem. 1971 Apr 25;246(8):2680-91 PMID: 4928649
  12. Endonuclease-I-deficient and ribonuclease I-deficient Escherichia coli mutants.
    J Mol Biol. 1968 Jul 14;34(2):331-46 PMID: 4938550
  13. The chromosome of bacteriophage T5. I. Analysis of the single-stranded DNA fragments by agarose gel electrophoresis.
    J Mol Biol. 1972 Feb 14;63(3):383-95 PMID: 5014925
  14. The chromosome of bacteriophage T5. II. Arrangement of the single-stranded DNA fragments in the T5 + and T5st(O) chromosomes.
    J Mol Biol. 1972 Feb 14;63(3):397-407 PMID: 5014926
  15. Location of single-strand interruptions in the DNA of bacteriophage T5.
    Proc Natl Acad Sci U S A. 1969 Apr;62(4):1167-74 PMID: 5256414
  16. A restriction enzyme from Hemophilus influenzae. II.
    J Mol Biol. 1970 Jul 28;51(2):393-409 PMID: 5312501
  17. Calibration of molecular weight scales for DNA.
    J Mol Biol. 1969 Dec 14;46(2):313-28 PMID: 5360042
  18. Analysis of the interruptions in bacteriophage T5 DNA.
    J Mol Biol. 1970 Feb 14;47(3):477-93 PMID: 5418167
  19. Molecular weights of coliphages and colip- hage DNA. II. Measurement of diffusion coefficients using optical mixing spectroscopy, and measurement of sedimentation coefficients.
    J Mol Biol. 1970 Dec 28;54(3):547-56 PMID: 5492019
  20. Mapping of deletions and substitutions in heteroduplex DNA molecules of bacteriophage lambda by electron microscopy.
    Science. 1969 Mar 21;163(3873):1343-8 PMID: 5765116
  21. Molecular weights of homogeneous coliphage DNA's from density-gradient sedimentation equilibrium.
    J Mol Biol. 1969 Aug 28;44(1):143-60 PMID: 5811826
  22. An exonuclease induced by bacteriophage lambda. II. Nature of the enzymatic reaction.
    J Biol Chem. 1967 Feb 25;242(4):679-86 PMID: 6017737
  23. A DEOXYRIBONUCLEIC ACID PHOSPHATASE-EXONUCLEASE FROM ESCHERICHIA COLI. I. PURIFICATION OF THE ENZYME AND CHARACTERIZATION OF THE PHOSPHATASE ACTIVITY.
    J Biol Chem. 1964 Jan;239:242-50 PMID: 14114850
  24. A DEOXYRIBONUCLEIC ACID PHOSPHATASE-EXONUCLEASE FROM ESCHERICHIA COLI. II. CHARACTERIZATION OF THE EXONUCLEASE ACTIVITY.
    J Biol Chem. 1964 Jan;239:251-8 PMID: 14114851
  25. THE ARRANGEMENTS OF NUCLEOTIDE SEQUENCES IN T2 AND T5 BACTERIOPHAGE DNA MOLECULES.
    Biophys J. 1964 Mar;4:93-106 PMID: 14130440
  26. Heteroduplex mapping of heat-resistant deletion mutants of bacteriophage t5.
    J Virol. 1975 May;15(5):1276-80 PMID: 16789160
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.23.3.725-736.1977
Published
1977-09-00
Pages
725-36
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC515885
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.23.3.725-736.1977 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2