Ribosomal abnormality in recA mutants of Escherichia coli.

Molecular & general genetics : MGG ·Vol. 154 ·No. 1 ·1977-07-07 ·Pages 83-6

Powell KA, Emmerson PT

Abstract

The tif-1 mutation has been shown to affect protein synthesis in vitro by increasing translational ambiguity (Ephrati-Elizur, Luther-Davies and Hayes, 1976). It is demonstrated here that some recA mutations confer similar abnormality. By comparing suitable combinations of ribosomes and soluble proteins from recA+ and recA cells the defect is shown to be associated with ribosomes. The recA mutation, which suppresses most phenotype characteristics of the tif-1 mutation (Castellazzi, George and Buttin, 1972(b)) does not suppress the ribosomal abnormality. Since the closely linked tif-1 and recA mutations lead to the expression of a common property they may be in the same gene.

MeSH Terms

Bacterial Proteins/biosynthesis Escherichia coli/metabolism Hot Temperature Mutation Phenotype RNA, Bacterial/metabolism RNA, Messenger/metabolism Recombination, Genetic Ribosomes/metabolism

Chemicals

Bacterial Proteins RNA, Bacterial RNA, Messenger

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Powell K A

Emmerson P T

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24 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00265580

Published

1977-07-07

Pages

83-6

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00265580

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Ribosomal abnormality in recA mutants of Escherichia coli.

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