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PMID: 331313 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Identification of spacer tRNA genes in individual ribosomal RNA transcription units of Escherichia coli.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 7 ·1977-07-00 ·Pages 2710-4

Morgan EA, Ikemura T, Nomura M

Abstract

Transfer RNA genes ("spacer tRNA genes") are present in the spacer region between 16S and 23S rRNA genes in Escherichia coli. We have analyzed spacer tRNA genes carried by seven rRNA operons with different chromosomal locations. Six of these were isolated on plasmids and one on a transducing phage. We found that, in addition to the two previously identified genes for tRNA2Glu and tRNAIIle, there is a spacer tRNA gene which codes for tRNAIBAla. Of the seven rRNA operons studied, three had both tRNAIBAla and tRNAIIle genes, and the remaining four had the tRNA2Glu gene in their spacers. In addition, genes for tRNAIAsp were found near the distal ends of two different rRNA operons.

MeSH Terms
Alanine Aspartic Acid Chromosome Mapping Escherichia coli Genes Glutamates Isoleucine Nucleic Acid Hybridization Operon Plasmids RNA, Bacterial RNA, Ribosomal RNA, Transfer
Chemicals
Glutamates RNA, Bacterial RNA, Ribosomal Isoleucine Aspartic Acid RNA, Transfer Alanine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Morgan E A
Ikemura T
Nomura M
References (15)
15 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.74.7.2710
Published
1977-07-00
Pages
2710-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC431257
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.74.7.2710 PMC Full Text View journal details
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