Home LiteratureArticle Details
PMID: 3313280 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

DNA synthesis is initiated at two positions within the origin of replication of plasmid R1162.

Nucleic acids research ·Vol. 15 ·No. 20 ·1987-10-26 ·Pages 8319-31

Lin LS, Meyer RJ

Abstract

DNA synthesis of broad host-range plasmid R1162 is initiated from two positions, flanking a large (40 bp stem, 40 bp loop) inverted repeat. Each start-point is located within a highly conserved, but oppositely oriented, 10 base-pair sequence. Synthesis from the two positions converges within the intervening inverted repeat. An analysis of deletions suggests that both start positions must be present for synthesis. A model describing possible early events in replication of plasmid R1162 is presented.

MeSH Terms
Base Sequence DNA Replication DNA, Bacterial/biosynthesis Escherichia coli/genetics Molecular Sequence Data Mutation Nucleic Acid Hybridization Plasmids
Chemicals
DNA, Bacterial
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lin L S
Department of Microbiology, University of Texas, Austin 78712.
Meyer R J
References (22)
22 references, click to expand
  1. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.
    Proc Natl Acad Sci U S A. 1972 Aug;69(8):2110-4 PMID: 4559594
  2. Copy-number of broad host-range plasmid R1162 is regulated by a small RNA.
    Nucleic Acids Res. 1986 Oct 24;14(20):8027-46 PMID: 2430262
  3. Plasmid ColEl as a molecular vehicle for cloning and amplification of DNA.
    Proc Natl Acad Sci U S A. 1974 Sep;71(9):3455-9 PMID: 4610576
  4. Comparison of the deoxyribonucleic acid molecular weights and homologies of plasmids conferring linked resistance to streptomycin and sulfonamides.
    J Bacteriol. 1974 Nov;120(2):618-30 PMID: 4616941
  5. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  6. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  7. Isolation and characterization of transducing coliphage fd carrying a kanamycin resistance gene.
    Gene. 1978 Feb;3(1):39-51 PMID: 344143
  8. Replication of the nonconjugative plasmid RSF1010 in Escherichia coli K-12.
    J Bacteriol. 1978 Jun;134(3):1117-22 PMID: 350840
  9. A system for shotgun DNA sequencing.
    Nucleic Acids Res. 1981 Jan 24;9(2):309-21 PMID: 6259625
  10. Purified bacteriophage lambda O protein binds to four repeating sequences at the lambda replication origin.
    Nucleic Acids Res. 1981 Apr 24;9(8):1789-99 PMID: 6264392
  11. A rapid boiling method for the preparation of bacterial plasmids.
    Anal Biochem. 1981 Jun;114(1):193-7 PMID: 6269464
  12. A procedure for the large-scale isolation of highly purified plasmid DNA using alkaline extraction and binding to glass powder.
    Anal Biochem. 1982 Apr;121(2):382-7 PMID: 6179438
  13. Replication of the broad host range plasmid RSF1010 in cell-free extracts of Escherichia coli and Pseudomonas aeruginosa.
    Nucleic Acids Res. 1982 Aug 11;10(15):4687-702 PMID: 6813825
  14. A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments.
    Gene. 1982 Oct;19(3):269-76 PMID: 6295880
  15. Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination.
    Proc Natl Acad Sci U S A. 1983 Jul;80(13):3963-5 PMID: 6575390
  16. Interaction of the plasmid R6K-encoded replication initiator protein with its binding sites on DNA.
    Cell. 1983 Aug;34(1):125-34 PMID: 6224568
  17. Replication of the broad host range plasmid RSF1010: requirement for three plasmid-encoded proteins.
    Proc Natl Acad Sci U S A. 1984 Feb;81(3):654-8 PMID: 6322159
  18. Plasmid P1 replication: negative control by repeated DNA sequences.
    Proc Natl Acad Sci U S A. 1984 Oct;81(20):6456-60 PMID: 6387706
  19. Protein RepC is involved in copy number control of the broad host range plasmid RSF1010.
    Proc Natl Acad Sci U S A. 1985 Sep;82(18):6090-4 PMID: 3898079
  20. Directly repeated, 20-bp sequence of plasmid R1162 DNA is required for replication, expression of incompatibility, and copy-number control.
    Plasmid. 1986 Jan;15(1):35-47 PMID: 3513218
  21. Mechanism of autonomous control of the Escherichia coli F plasmid: different complexes of the initiator/repressor protein are bound to its operator and to an F plasmid replication origin.
    Nucleic Acids Res. 1986 Jul 25;14(14):5693-711 PMID: 3016662
  22. Construction of biologically functional bacterial plasmids in vitro.
    Proc Natl Acad Sci U S A. 1973 Nov;70(11):3240-4 PMID: 4594039
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.20.8319
Published
1987-10-26
Pages
8319-31
Language
English
Region
England
NLM ID
0411011
PMCID
PMC306362
Subset
IM
Grants
NIGMS NIH HHS · GM36822 · United States
External Links
PubMed source DOI: 10.1093/nar/15.20.8319 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2