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PMID: 3313392 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Naturally occurring anti-band-3 antibodies and complement together mediate phagocytosis of oxidatively stressed human erythrocytes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 21 ·1987-11-00 ·Pages 7368-72

Lutz HU, Bussolino F, Flepp R, Fasler S, Stammler P, Kazatchkine MD, Arese P

Abstract

Treatment of erythrocytes with the thiol-specific oxidant azodicarboxylic acid bis(dimethylamide) (diamide) enhances their phagocytosis by adherent monocytes. Phagocytosis of diamide-treated erythrocytes required that the cells were opsonized with whole serum, since complement inactivation abolished phagocytosis. Opsonization with whole serum containing 20-100 times the physiological concentration of naturally occurring anti-band-3 antibodies enhanced phagocytosis of diamide-treated erythrocytes. High inputs of anti-band-3 also restored phagocytosis of erythrocytes that had been incubated with complement-inactivated serum. Elevated concentrations of anti-spectrin antibodies were ineffective in whole and complement-inactivated serum. Specific recognition of diamide-treated erythrocytes by anti-band-3 antibodies may be due to generation of anti-band-3 reactive protein oligomers on intact diamide-treated erythrocytes. Generation of such oligomers was dose-dependent with respect to diamide. Bound anti-band-3 alone was not sufficient to mediate phagocytosis. It resulted in deposition of complement component C3b on the cells through activation of the alternative complement pathway in amounts exceeding that of bound antibodies by two orders of magnitude. Thus, anti-band-3 and complement together mediate phagocytosis of oxidatively stressed erythrocytes, which stimulate senescent erythrocytes with respect to bound antibody and complement.

MeSH Terms
Anion Exchange Protein 1, Erythrocyte/immunology,physiology Antibodies Antigen-Antibody Complex Complement C3b/metabolism Diamide/pharmacology Erythrocytes/drug effects,immunology,physiology Humans Kinetics Macrophage-1 Antigen Phagocytosis Receptors, Complement/metabolism
Chemicals
Anion Exchange Protein 1, Erythrocyte Antibodies Antigen-Antibody Complex Macrophage-1 Antigen Receptors, Complement Diamide Complement C3b
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Lutz H U
Department of Biochemistry, Swiss Federal Institute of Technology, Zurich.
Bussolino F
Flepp R
Fasler S
Stammler P
Kazatchkine M D
Arese P
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.21.7368
Published
1987-11-00
Pages
7368-72
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC299297
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.84.21.7368 PMC Full Text View journal details
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