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In vitro mutagenesis.
Annu Rev Genet. 1985;19:423-62
PMID: 3002241
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Crystal structure of a deletion mutant of a tyrosyl-tRNA synthetase complexed with tyrosine.
J Mol Biol. 1987 Mar 20;194(2):287-97
PMID: 3612807
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Structural heterogeneity in protein crystals.
Biochemistry. 1986 Sep 9;25(18):5018-27
PMID: 3768328
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Site-directed mutagenesis reveals role of mobile arginine residue in lactate dehydrogenase catalysis.
Nature. 1986 Dec 18-31;324(6098):699-702
PMID: 3796734
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Internal structural features of E. coli glycyl-tRNA synthetase examined by subunit polypeptide chain fusions.
J Biol Chem. 1986 May 25;261(15):6643-6
PMID: 3009467
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Site-directed mutagenesis of cytochrome c shows that an invariant Phe is not essential for function.
Nature. 1985 Jan 10-18;313(5998):152-4
PMID: 2981414
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Amino acid replacements in yeast iso-1-cytochrome c. Comparison with the phylogenetic series and the tertiary structure of related cytochromes c.
J Biol Chem. 1986 Mar 5;261(7):3259-71
PMID: 3005287
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Changing the binding specificity of a repressor by redesigning an alpha-helix.
Nature. 1985 Aug 15-21;316(6029):601-5
PMID: 4033758
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Tertiary templates for proteins. Use of packing criteria in the enumeration of allowed sequences for different structural classes.
J Mol Biol. 1987 Feb 20;193(4):775-91
PMID: 2441069
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Tinkering with enzymes: what are we learning?
Science. 1987 Jun 5;236(4806):1252-8
PMID: 3296192
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Site-directed mutagenesis of an invariant amino acid residue at the variable-diversity segments junction of an antibody.
Proc Natl Acad Sci U S A. 1986 Apr;83(8):2628-31
PMID: 3085101
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A protein structure from nuclear magnetic resonance data. lac repressor headpiece.
J Mol Biol. 1985 Mar 5;182(1):179-82
PMID: 3889346
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Site-directed mutagenesis shows that tyrosine 248 of carboxypeptidase A does not play a crucial role in catalysis.
Nature. 1985 Oct 10-16;317(6037):551-5
PMID: 3840231
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The structure of cytochrome c and its relation to recent studies of long-range electron transfer.
Protein Eng. 1987 Feb-Mar;1(2):83-8
PMID: 2853357
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Site-specific mutagenesis of the alpha-helices of calmodulin. Effects of altering a charge cluster in the helix that links the two halves of calmodulin.
J Biol Chem. 1987 Mar 5;262(7):3278-84
PMID: 3029108
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Effects of site-specific amino acid modification on protein interactions and biological function.
Annu Rev Biochem. 1985;54:597-629
PMID: 3896127
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Yeast cytochrome c with phenylalanine or tyrosine at position 87 transfers electrons to (zinc cytochrome c peroxidase)+ at a rate ten thousand times that of the serine-87 or glycine-87 variants.
Proc Natl Acad Sci U S A. 1987 Mar;84(5):1249-52
PMID: 3029774
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Effects of the phenylalanine-22----leucine, glutamic acid-49----methionine, glycine-234----aspartic acid, and glycine-234----lysine mutations on the folding and stability of the alpha subunit of tryptophan synthase from Escherichia coli.
Biochemistry. 1986 May 20;25(10):2965-74
PMID: 2872918
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Toward computer-aided site-directed mutagenesis of enzymes.
Proc Natl Acad Sci U S A. 1986 Jun;83(11):3806-10
PMID: 3520555
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Unpaired cysteine-54 interferes with the ability of an engineered disulfide to stabilize T4 lysozyme.
Biochemistry. 1986 Feb 11;25(3):733-9
PMID: 3513834
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Protein engineering.
Protein Eng. 1986 Oct-Nov;1(1):7-16
PMID: 3333843
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Specificity of tRNA charging in Tyr-tRNA synthetase.
Protein Eng. 1986 Oct-Nov;1(1):5-6
PMID: 3507687
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Protein engineering by chemical means?
Protein Eng. 1987 Jun;1(3):151-7
PMID: 3333844
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The cloning and expression of an anti-peptide antibody: a system for rapid analysis of the binding properties of engineered antibodies.
Protein Eng. 1986 Oct-Nov;1(1):59-65
PMID: 3507688
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Folding of dihydrofolate reductase from Escherichia coli.
Biochemistry. 1986 Sep 23;25(19):5445-52
PMID: 3535877
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Probing steric and hydrophobic effects on enzyme-substrate interactions by protein engineering.
Science. 1986 Aug 8;233(4764):659-63
PMID: 17835820
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The crystallographically determined structures of atypical strained disulfides engineered into subtilisin.
J Biol Chem. 1986 Nov 25;261(33):15480-5
PMID: 3096989
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Modification of the active site of alkaline phosphatase by site-directed mutagenesis.
Science. 1986 Jan 10;231(4734):145-8
PMID: 3510454
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Genetic analysis of staphylococcal nuclease: identification of three intragenic "global" suppressors of nuclease-minus mutations.
Genetics. 1985 Aug;110(4):539-55
PMID: 3896923
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Semisynthesis of horse heart cytochrome c analogues from two or three fragments.
Proc Natl Acad Sci U S A. 1985 Dec;82(24):8279-83
PMID: 3001693
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Altered specificities of genetically engineered alpha 1 antitrypsin variants.
Protein Eng. 1986 Oct-Nov;1(1):29-35
PMID: 3509863
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Oligonucleotide-directed mutagenesis as a general and powerful method for studies of protein function.
Proc Natl Acad Sci U S A. 1982 Nov;79(21):6409-13
PMID: 6983070
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Activation of complement by immunoglobulin M is impaired by the substitution serine-406----asparagine in the immunoglobulin mu heavy chain.
Proc Natl Acad Sci U S A. 1986 Oct;83(20):7678-82
PMID: 3094013
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The design, synthesis, and crystallization of an alpha-helical peptide.
Proteins. 1986 Sep;1(1):16-22
PMID: 3449847
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Site-directed mutagenesis.
Biochem J. 1986 Jul 1;237(1):1-7
PMID: 3541892
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Function of Lys-166 of Rhodospirillum rubrum ribulosebisphosphate carboxylase/oxygenase as examined by site-directed mutagenesis.
J Biol Chem. 1987 Mar 15;262(8):3496-501
PMID: 3102487
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Designing substrate specificity by protein engineering of electrostatic interactions.
Proc Natl Acad Sci U S A. 1987 Mar;84(5):1219-23
PMID: 3547407
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Substitutions of proline 76 in yeast iso-1-cytochrome c. Analysis of residues compatible and incompatible with folding requirements.
J Biol Chem. 1985 Oct 25;260(24):13225-36
PMID: 2997158
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Structure and mechanism of copper, zinc superoxide dismutase.
Nature. 1983 Nov 17-23;306(5940):284-7
PMID: 6316150
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Evaluation and improvements in the automatic alignment of protein sequences.
Protein Eng. 1987 Feb-Mar;1(2):89-94
PMID: 3507699
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Yeast cytochrome c peroxidase: mutagenesis and expression in Escherichia coli show tryptophan-51 is not the radical site in compound I.
Biochemistry. 1987 Jan 27;26(2):351-60
PMID: 3030406
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Recombinant DNA-derived forms of human alpha 1-proteinase inhibitor. Studies on the alanine 358 and cysteine 358 substituted mutants.
J Biol Chem. 1986 Aug 5;261(22):10404-9
PMID: 3525544
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Genetic studies on protein export in bacteria.
Curr Top Microbiol Immunol. 1986;125:5-27
PMID: 3091324
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Regeneration of active enzyme by formation of hybrids from inactive derivatives: implications for active sites shared between polypeptide chains of aspartate transcarbamoylase.
Proc Natl Acad Sci U S A. 1985 Jan;82(2):361-5
PMID: 3881763
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Conserved protein domains in a myosin heavy chain gene from Dictyostelium discoideum.
Proc Natl Acad Sci U S A. 1986 Dec;83(24):9433-7
PMID: 3540939
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Dihydrofolate reductase. 1H resonance assignments and coenzyme-induced conformational changes.
J Mol Biol. 1986 Mar 5;188(1):81-97
PMID: 3012098
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Protein engineering of cytochrome c by semisynthesis: substitutions at glutamic acid 66.
Protein Eng. 1986 Oct-Nov;1(1):23-7
PMID: 2907133
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Replacing the complementarity-determining regions in a human antibody with those from a mouse.
Nature. 1986 May 29-Jun 4;321(6069):522-5
PMID: 3713831
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Cloning, sequencing, and secretion of Bacillus amyloliquefaciens subtilisin in Bacillus subtilis.
Nucleic Acids Res. 1983 Nov 25;11(22):7911-25
PMID: 6316278
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Bovine chymotrypsinogen A X-ray crystal structure analysis and refinement of a new crystal form at 1.8 A resolution.
J Mol Biol. 1985 Oct 5;185(3):595-624
PMID: 4057257
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The relation between the divergence of sequence and structure in proteins.
EMBO J. 1986 Apr;5(4):823-6
PMID: 3709526
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Oxygen binding properties of human mutant hemoglobins synthesized in Escherichia coli.
Proc Natl Acad Sci U S A. 1985 Nov;82(21):7252-5
PMID: 3903751
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The conformations of hirudin in solution: a study using nuclear magnetic resonance, distance geometry and restrained molecular dynamics.
EMBO J. 1987 Feb;6(2):529-37
PMID: 15981339
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Synergism in folding of a double mutant of the alpha subunit of tryptophan synthase.
Biochemistry. 1986 Oct 21;25(21):6356-60
PMID: 3539187
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Crystallographic characterization of recombinant human CuZn superoxide dismutase.
J Biol Chem. 1986 Dec 5;261(34):16215-8
PMID: 3782115
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Strategies and applications of in vitro mutagenesis.
Science. 1985 Sep 20;229(4719):1193-201
PMID: 2994214
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Use of binding energy in catalysis analyzed by mutagenesis of the tyrosyl-tRNA synthetase.
Biochemistry. 1986 Apr 22;25(8):1881-6
PMID: 3518794
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Determination and analysis of the 2 A-structure of copper, zinc superoxide dismutase.
J Mol Biol. 1982 Sep 15;160(2):181-217
PMID: 7175933
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Redesigning trypsin: alteration of substrate specificity.
Science. 1985 Apr 19;228(4697):291-7
PMID: 3838593
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Construction of heterodimer tyrosyl-tRNA synthetase shows tRNATyr interacts with both subunits.
Proc Natl Acad Sci U S A. 1986 Mar;83(5):1189-92
PMID: 3006039
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Computer-aided model-building strategies for protein design.
Biochemistry. 1986 Oct 7;25(20):5987-91
PMID: 3790501
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Calculation of protein conformations by proton-proton distance constraints. A new efficient algorithm.
J Mol Biol. 1985 Dec 5;186(3):611-26
PMID: 2419572
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Effect of a single amino acid substitution on the folding of the alpha subunit of tryptophan synthase.
Biochemistry. 1983 Mar 15;22(6):1445-52
PMID: 6132619
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Three-dimensional structure of ribulose-1,5-bisphosphate carboxylase/oxygenase from Rhodospirillum rubrum at 2.9 A resolution.
EMBO J. 1986 Dec 20;5(13):3409-15
PMID: 16453738
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Mutational analysis of protein folding pathways: the P22 tailspike endorhamnosidase.
Methods Enzymol. 1986;131:250-66
PMID: 3773760
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Internal thermodynamics of position 51 mutants and natural variants of tyrosyl-tRNA synthetase.
Biochemistry. 1986 Apr 22;25(8):1891-7
PMID: 3518795
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Nonessentiality of histidine 291 of Rhodospirillum rubrum ribulose-bisphosphate carboxylase/oxygenase as determined by site-directed mutagenesis.
J Biol Chem. 1986 Aug 5;261(22):10087-92
PMID: 3090029
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Three-dimensional structure of proteins determined by molecular dynamics with interproton distance restraints: application to crambin.
Proc Natl Acad Sci U S A. 1986 Jun;83(11):3801-5
PMID: 3459158
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Kinetic and magnetic resonance studies of active-site mutants of staphylococcal nuclease: factors contributing to catalysis.
Biochemistry. 1987 Mar 10;26(5):1289-300
PMID: 3567171
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Synthesis and use of synthetic oligonucleotides.
Annu Rev Biochem. 1984;53:323-56
PMID: 6383196
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The nature of enzyme catalysis in trypsin.
Proc Natl Acad Sci U S A. 1986 Feb;83(3):649-53
PMID: 3456162
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Transition-state stabilization in the mechanism of tyrosyl-tRNA synthetase revealed by protein engineering.
Proc Natl Acad Sci U S A. 1985 Dec;82(23):7840-4
PMID: 3865201
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Subtilisin; a stereochemical mechanism involving transition-state stabilization.
Biochemistry. 1972 Nov 7;11(23):4293-303
PMID: 5079900
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Secretion and autoproteolytic maturation of subtilisin.
Proc Natl Acad Sci U S A. 1986 May;83(10):3096-100
PMID: 3517850
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An engineered intersubunit disulfide enhances the stability and DNA binding of the N-terminal domain of lambda repressor.
Biochemistry. 1986 Oct 7;25(20):5992-8
PMID: 3539184
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Mutant forms of staphylococcal nuclease with altered patterns of guanidine hydrochloride and urea denaturation.
Proteins. 1986 Sep;1(1):81-9
PMID: 3449854
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Synthesis in E. coli of alpha 1-antitrypsin variants of therapeutic potential for emphysema and thrombosis.
Nature. 1985 Jan 10-18;313(5998):149-51
PMID: 3880873
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Folding/unfolding kinetics of mutant forms of iso-1-cytochrome c with replacement of proline-71.
Biochemistry. 1986 Nov 4;25(22):6959-64
PMID: 3026440
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Yeast phosphoglycerate kinase: investigation of catalytic function by site-directed mutagenesis.
Biochem J. 1987 Jan 15;241(2):609-14
PMID: 3297036
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Isolation and properties of recombinant DNA produced variants of human alpha 1-proteinase inhibitor.
J Biol Chem. 1985 Apr 10;260(7):4384-9
PMID: 3872299
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Dynamics of proteins: elements and function.
Annu Rev Biochem. 1983;52:263-300
PMID: 6351724
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Recombinant antibodies possessing novel effector functions.
Nature. 1984 Dec 13-19;312(5995):604-8
PMID: 6095112
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Site-directed mutagenesis and the role of the oxyanion hole in subtilisin.
Proc Natl Acad Sci U S A. 1986 Jun;83(11):3743-5
PMID: 3520553
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Human alpha 1-proteinase inhibitor. Crystal structure analysis of two crystal modifications, molecular model and preliminary analysis of the implications for function.
J Mol Biol. 1984 Aug 15;177(3):531-57
PMID: 6332197
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Nuclear magnetic resonance study of the state of protonation of inhibitors bound to mutant dihydrofolate reductase lacking the active-site carboxyl.
Biochemistry. 1986 Nov 4;25(22):7229-35
PMID: 3542023
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Active human-yeast chimeric phosphoglycerate kinases engineered by domain interchange.
Science. 1986 Aug 15;233(4765):788-90
PMID: 3526552
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Construction and characterization of an active factor VIII variant lacking the central one-third of the molecule.
Biochemistry. 1986 Dec 30;25(26):8343-7
PMID: 3030393
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Importance of a hydrophobic residue in binding and catalysis by dihydrofolate reductase.
Proc Natl Acad Sci U S A. 1986 Oct;83(20):7718-20
PMID: 3463995
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Thiol-beta-lactamase: replacement of the active-site serine of RTEM beta-lactamase by a cysteine residue.
Proc Natl Acad Sci U S A. 1982 Dec;79(23):7157-60
PMID: 6818541
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Electrostatic recognition between superoxide and copper, zinc superoxide dismutase.
Nature. 1983 Nov 17-23;306(5940):287-90
PMID: 6646211
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Isolation of superoxide dismutase mutants in Escherichia coli: is superoxide dismutase necessary for aerobic life?
EMBO J. 1986 Mar;5(3):623-30
PMID: 3011417
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In vivo formation and stability of engineered disulfide bonds in subtilisin.
J Biol Chem. 1986 May 15;261(14):6564-70
PMID: 3516996
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The purification of eukaryotic polypeptides synthesized in Escherichia coli.
Biochem J. 1986 Nov 15;240(1):1-12
PMID: 3548705
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Folding pathway of a circular form of bovine pancreatic trypsin inhibitor.
J Mol Biol. 1984 Nov 5;179(3):527-45
PMID: 6210371
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Synthesis in yeast of a functional oxidation-resistant mutant of human alpha-antitrypsin.
Nature. 1984 Nov 1-7;312(5989):77-80
PMID: 6387509
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A study on the role of evolutionarily invariant leucine 32 of cytochrome c.
J Biol Chem. 1986 Feb 25;261(6):2697-711
PMID: 3005260
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An engineered disulfide bond in dihydrofolate reductase.
Biochemistry. 1987 Apr 21;26(8):2182-9
PMID: 3304420
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Functional role of aspartic acid-27 in dihydrofolate reductase revealed by mutagenesis.
Science. 1986 Mar 7;231(4742):1123-8
PMID: 3511529
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Tyrosyl-tRNA synthetase forms a mononucleotide-binding fold.
J Mol Biol. 1982 Jul 15;158(4):699-709
PMID: 7120416
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Mutation of antitrypsin to antithrombin. alpha 1-antitrypsin Pittsburgh (358 Met leads to Arg), a fatal bleeding disorder.
N Engl J Med. 1983 Sep 22;309(12):694-8
PMID: 6604220
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Use of directed mutagenesis to probe the role of tyrosine 198 in the catalytic mechanism of carboxypeptidase A.
J Biol Chem. 1987 Jan 15;262(2):576-82
PMID: 3542991
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Selective alteration of substrate specificity by replacement of aspartic acid-189 with lysine in the binding pocket of trypsin.
Biochemistry. 1987 May 5;26(9):2616-23
PMID: 3111531
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A model of synthetase/transfer RNA interaction as deduced by protein engineering.
Nature. 1986 Mar 27-Apr 2;320(6060):371-3
PMID: 3960121
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Modular arrangement of functional domains along the sequence of an aminoacyl tRNA synthetase.
Nature. 1983 Dec 1-7;306(5942):441-7
PMID: 6358898
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Studies of the radical species in compound ES of cytochrome c peroxidase altered by site-directed mutagenesis.
Proc Natl Acad Sci U S A. 1986 Mar;83(5):1295-9
PMID: 3006043
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Refined crystal structures of Escherichia coli and chicken liver dihydrofolate reductase containing bound trimethoprim.
J Biol Chem. 1985 Jan 10;260(1):381-91
PMID: 3880742
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Reaction energetics of a mutant triosephosphate isomerase in which the active-site glutamate has been changed to aspartate.
Biochemistry. 1986 Nov 4;25(22):7142-54
PMID: 2879556
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Direct determination of the protonation states of aspartic acid-102 and histidine-57 in the tetrahedral intermediate of the serine proteases: neutron structure of trypsin.
Biochemistry. 1981 Oct 27;20(22):6462-74
PMID: 7030393
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Free energy of hydrolysis of tyrosyl adenylate and its binding to wild-type and engineered mutant tyrosyl-tRNA synthetases.
Biochemistry. 1986 Oct 21;25(21):6603-8
PMID: 3466647
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Control of oligomeric enzyme thermostability by protein engineering.
Proc Natl Acad Sci U S A. 1987 Feb;84(3):675-9
PMID: 3543933
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Directed mutagenesis of dihydrofolate reductase.
Science. 1983 Nov 18;222(4625):782-8
PMID: 6356360
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The design and production of semisynthetic ribonucleases with increased thermostability by incorporation of S-peptide analogues with enhanced helical stability.
Proteins. 1986 Sep;1(1):23-33
PMID: 3449849
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Use of site-directed mutagenesis to study the mechanism of a membrane transport protein.
Biochemistry. 1987 Apr 21;26(8):2071-6
PMID: 3304418
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Deamidation, isomerization, and racemization at asparaginyl and aspartyl residues in peptides. Succinimide-linked reactions that contribute to protein degradation.
J Biol Chem. 1987 Jan 15;262(2):785-94
PMID: 3805008
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Shared active sites in oligomeric enzymes: model studies with defective mutants of aspartate transcarbamoylase produced by site-directed mutagenesis.
Proc Natl Acad Sci U S A. 1987 Jan;84(1):31-5
PMID: 3540957