Home LiteratureArticle Details
PMID: 33152 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Isolation, characterization, and crystallization of ribulosebisphosphate carboxylase from autotrophically grown Rhodospirillum rubrum.

Journal of bacteriology ·Vol. 137 ·No. 1 ·1979-01-00 ·Pages 490-501

Schloss JV, Phares EF, Long MV, Norton IL, Stringer CD, Hartman FC

Abstract

Serial culture of Rhodospirillum rubrum with 2% CO2 in H2 as the exclusive carbon source resulted in a rather large fraction of the soluble protein (greater than 40%) being comprised of ribulosebisphosphate carboxylase (about sixfold higher than the highest value previously reported). Isolation of the enzyme from these cells revealed that it has physical and kinetic properties similar to those previously described for the enzyme derived from cells grown on butyrate. Notably, the small subunit (which is a constituent of the carboxylase from eucaryotes and most procaryotes) was absent in the enzyme from autotrophically grown R. rubrum. Edman degradation of the purified enzyme revealed that the NH2 terminus is free (in contrast to the catalytic subunit of the carboxylase from eucaryotes) and that the NH2-terminal sequence is Met-Asp-Gln-Ser-Ser-Arg-Tyr-Val-Asn-Leu-Ala-Leu-Lys-Glu-Glu-Asp-Leu-Ile-Ala-Gly-Gly-Glx-His-Val-Leu-. Crystals of the enzyme were readily obtained by dialysis against distilled water.

MeSH Terms
Amino Acids/analysis Carboxy-Lyases/metabolism Crystallization Freezing Hydrogen-Ion Concentration Kinetics Magnesium/pharmacology Rhodospirillum rubrum/enzymology Ribulose-Bisphosphate Carboxylase/analysis,metabolism Ultracentrifugation
Chemicals
Amino Acids Carboxy-Lyases Ribulose-Bisphosphate Carboxylase Magnesium
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Schloss J V
Phares E F
Long M V
Norton I L
Stringer C D
Hartman F C
References (59)
59 references, click to expand
  1. The activation of ribulose-1,5-bisphosphate carboxylase by carbon dioxide and magnesium ions. Equilibria, kinetics, a suggested mechanism, and physiological implications.
    Biochemistry. 1976 Feb 10;15(3):529-36 PMID: 3199
  2. A model for the kinetics of activation and catalysis of ribulose 1,5-bisphosphate carboxylase.
    Biochem J. 1976 Dec 1;159(3):563-70 PMID: 12741
  3. Different molecular forms of D-ribulose-1,5-bisphosphate carboxylase from Rhodopseudomonas sphaeroides.
    J Biol Chem. 1977 Feb 10;252(3):943-9 PMID: 14141
  4. Isolation and preliminary characterization of two forms of ribulose 1,5-bisphosphate carboxylase from Rhodopseudomonas capsulata.
    J Bacteriol. 1977 Dec;132(3):818-23 PMID: 21872
  5. Modification of Rhodospirillum rubrum ribulose bisphosphate carboxylase with pyridoxal phosphate. 1. Identification of a lysyl residue at the active site.
    Biochemistry. 1978 Apr 4;17(7):1282-7 PMID: 26381
  6. Origins of prokaryotes, eukaryotes, mitochondria, and chloroplasts.
    Science. 1978 Jan 27;199(4327):395-403 PMID: 202030
  7. Ribulose diphosphate carboxylase/oxygenase. III. Isolation and properties.
    J Biol Chem. 1975 Jun 10;250(11):4229-33 PMID: 236314
  8. Regeneration of amino acids from thiazolinones formed in the Edman degradation.
    Anal Biochem. 1975 Sep;68(1):47-53 PMID: 242232
  9. Reaction of ribulosebisphosphate carboxylase from rhodospirlilum rubrum with the potential affinity label 3-bromo-1,4-dihydroxy-2-butanone 1,4-biphosphate.
    Biochem Biophys Res Commun. 1977 Mar 21;75(2):320-8 PMID: 403917
  10. Modification of Rhodospirillum rubrum ribulose bisphosphate carboxylase with pyridoxal phosphate. 2. Stoichiometry and kinetics of inactivation.
    Biochemistry. 1978 Apr 4;17(7):1288-93 PMID: 418800
  11. Sequences of two active-site peptides from spinach ribulosebisphosphate carboxylase/oxygenase.
    Biochem Biophys Res Commun. 1978 Feb 28;80(4):1043-8 PMID: 637859
  12. Ribulose bisphosphate carboxylase from methanol-grown Paracoccus denitrificans.
    J Bacteriol. 1978 Jun;134(3):1123-32 PMID: 659365
  13. Identification of essential lysyl and cysteinyl residues in spinach ribulosebisphosphate carboxylase/oxygenase modified by the affinity label N-bromoacetylethanolamine phosphate.
    J Biol Chem. 1978 Aug 25;253(16):5707-11 PMID: 670222
  14. Carbon dioxide assimilation in blue-green algae: initial studies on the structure of ribulose 1,5-bisphosphate carboxylase.
    J Bacteriol. 1976 Feb;125(2):531-9 PMID: 812868
  15. Purification, quaternary structure, composition, and properties of D-ribulose-1,5-bisphosphate carboxylase from Thiobacillus intermedius.
    J Bacteriol. 1976 Jul;127(1):505-15 PMID: 819423
  16. Essential primary amino groups of ribulose bisphosphate carboxylase/oxygenase indicated by reaction with pyridoxal 5'-phosphate.
    Arch Biochem Biophys. 1977 Feb;179(1):279-88 PMID: 843087
  17. D-Ribulose-1,5-bisphosphate carboxylase-oxygenase. Improved methods for the activation and assay of catalytic activities.
    Anal Biochem. 1977 Mar;78(1):66-75 PMID: 848758
  18. Regulation of ribulose 1,5-bisphosphate carboxylase-oxygenase activities by temperature pretreatment and chloroplast metabolites.
    Arch Biochem Biophys. 1976 Sep;176(1):344-51 PMID: 970962
  19. Pathways of carbon fixation in green plants.
    Annu Rev Biochem. 1975;44:123-45 PMID: 1094907
  20. The structure of form I crystals of D-ribulose-1,5-diphosphate carboxylase.
    J Mol Biol. 1975 Feb 5;91(4):391-9 PMID: 1152035
  21. Synthesis and characterization of 3-bromo-1,4-dihydroxy-2-butanone 1,4-bisphosphate, a potential affinity label for enzymes that bind sugar bisphosphates.
    J Org Chem. 1975 Sep 5;40(18):2638-42 PMID: 1159564
  22. Evidence for essential lysyl residues in ribulosebisphosphate carboxylase by use of the affinity label 3-bromo-1,4-dihydroxy-2-butanone 1,4-bisphosphate.
    J Biol Chem. 1975 Oct 25;250(20):8062-8 PMID: 1176459
  23. D-ribulose 1,5-diphosphate carboxylase from Rhodospirillum rubrum. I. Levels, purification, and effects of metallic ions.
    J Biol Chem. 1974 Jun 10;249(11):3453-8 PMID: 4208661
  24. D-ribulose 1,5-diphosphate carboxylase from Rhodospirillum rubrum. II. Quaternary structure, composition, catalytic, and immunological properties.
    J Biol Chem. 1974 Jun 10;249(11):3459-64 PMID: 4208662
  25. Studies on spinach leaf ribulosebisphosphate carboxylase. Carboxylase and oxygenase reaction examined by immunochemical methods.
    Biochemistry. 1974 May 21;13(11):2277-81 PMID: 4208775
  26. Ribulose diphosphate oxygenase. V. Presence in ribulose diphosphate carboxylase from Rhodospirillum rubrum.
    Biochem Biophys Res Commun. 1974 Aug 19;59(4):1233-41 PMID: 4213364
  27. D-ribulose 1, 5-diphosphate carboxylase from blue-green algae.
    Biochem Biophys Res Commun. 1974 Nov 6;61(1):45-52 PMID: 4216351
  28. Estimation of the molecular weight of ribulose diphosphate carboxylase sub-units.
    Biochem Biophys Res Commun. 1970 Jun 5;39(5):923-9 PMID: 4316432
  29. A new acid hydrolysis method for determining tryptophan in peptides and proteins.
    Anal Biochem. 1974 Jul;60(1):45-50 PMID: 4368334
  30. D-ribulose-1, 5-diphosphate carboxylase and the evolution of autotrophy.
    Biosystems. 1974 Oct;6(2):93-112 PMID: 4613398
  31. Interaction of ribulose diphosphate carboxylase with 2-carboxyribitol diphosphate, an analogue of the proposed carboxylated intermediate in the CO 2 fixation reaction.
    Biochem Biophys Res Commun. 1972 Aug 7;48(3):508-16 PMID: 4625865
  32. Regulation of ribulose-1,5-diphosphate carboxylase by 6-phospho-D-gluconate.
    Biochem Biophys Res Commun. 1972 Sep 5;48(5):1153-9 PMID: 4626611
  33. Photosynthetic carbon dioxide assimilation by Rhodospirillum rubrum.
    Arch Mikrobiol. 1973;88(3):213-23 PMID: 4630715
  34. Ribulose diphosphate oxygenase. II. Further proof of reaction products and mechanism of action.
    Biochemistry. 1973 Jan 2;12(1):18-23 PMID: 4683482
  35. Thin-layer chromatography of sub-nanomole amounts of phenylthiohydantoin (PTH) amino acids on polyamide sheets.
    Anal Biochem. 1973 Jun;53(2):624-8 PMID: 4716391
  36. Catalytic role of subunit A in ribulose-1,5-diphosphate carboxylase from Chromatium strain D.
    Arch Biochem Biophys. 1973 Jul;157(1):303-8 PMID: 4716958
  37. Oxidative formation of phosphoglycolate from ribulose-1,5-diphosphate catalysed by Chromatium ribulose-1,5-diphosphate carboxylase.
    Biochem Biophys Res Commun. 1973 Aug 21;53(4):1173-9 PMID: 4748814
  38. Further proof for the catalytic role of the larger subunit in the spinach leaf ribulose-1,5-diphosphate carboxylase.
    Biochem Biophys Res Commun. 1973 Oct 1;54(3):842-8 PMID: 4753193
  39. D-ribulose-1,5-bisphosphate carboxylase in Chlorobium thiosulfatophilum Tassajara.
    Biochim Biophys Acta. 1974 Mar 21;341(1):187-94 PMID: 4828843
  40. Crystalline fraction I protein: preparation in large yield.
    Science. 1972 Jun 9;176(4039):1145-6 PMID: 5035475
  41. Oxygen inhibition and other properties of soybean ribulose 1,5-diphosphate carboxylase.
    J Biol Chem. 1972 Apr 10;247(7):2171-6 PMID: 5062819
  42. Quantitative procedures for use with the Edman-Begg sequenator. Partial sequences of two unusual immunoglobulin light chains, Rzf and Sac.
    Biochemistry. 1971 Dec 21;10(26):4912-21 PMID: 5134536
  43. A new convenient method for estimation of total cystine-cysteine in proteins.
    Anal Biochem. 1969 Oct 15;32(1):185-90 PMID: 5398264
  44. X-ray diffraction studies of enzymes.
    Annu Rev Biochem. 1970;39:63-100 PMID: 5479039
  45. Studies on fraction-I protein. I. Effect of crystallization of fraction-I protein from tobacco leaves on ribulose diphosphate carboxylase activity.
    Biochim Biophys Acta. 1971 Jan 19;229(1):240-9 PMID: 5543610
  46. Reversible cold inactivation and heat reactivation of RuDP carboxylase activity of crystallized tobacco fraction I protein.
    Biochem Biophys Res Commun. 1971 Feb 19;42(4):664-8 PMID: 5543949
  47. Molecular diversity of the ribulose-1,5-diphosphate carboxylase from photosynthetic microorganisms.
    Science. 1968 Aug 2;161(3840):482-5 PMID: 5659689
  48. Photosynthesis in Rhodospirillum rubrum. IV. Isolation and characterization of ribulose 1,5-diphosphate carboxylase.
    J Biol Chem. 1969 Jun 25;244(12):3105-9 PMID: 5792651
  49. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
    J Biol Chem. 1969 Aug 25;244(16):4406-12 PMID: 5806584
  50. Structure and function of chloroplast proteins. 8. Some properties of ribulose-1,5-diphosphate carboxylase of athiorhodaceae in comparison with those of plant enzyme.
    Arch Biochem Biophys. 1969 Jun;132(1):255-61 PMID: 5816149
  51. Partial purification and characterization of two fructose diphosphate aldolases from Chlamydomonas mundana.
    Biochim Biophys Acta. 1967 Jan 11;132(1):145-54 PMID: 6030343
  52. Photosynthesis in Rhodospirillum rubrum. 3. Metabolic control of reductive pentose phosphate and tricarboxylic acid cycle enzymes.
    Plant Physiol. 1967 Apr;42(4):497-509 PMID: 6042359
  53. Tissue sulfhydryl groups.
    Arch Biochem Biophys. 1959 May;82(1):70-7 PMID: 13650640
  54. Light-dependent utilization of organic compounds and photoproduction of molecular hydrogen by photosynthetic bacteria; relationships with nitrogen metabolism.
    Arch Biochem Biophys. 1961 Sep;94:449-63 PMID: 13731247
  55. MEASUREMENT OF LOW ENERGY BETA-EMITTERS IN AQUEOUS SOLUTION BY LIQUID SCINTILLATION COUNTING OF EMULSIONS.
    Anal Chem. 1965 Jun;37:854-7 PMID: 14326976
  56. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  57. Origin of sequence heterogeneity of the small subunit of fraction 1 protein from Nicotiana tabacum.
    Biochem Biophys Res Commun. 1976 Dec 20;73(4):993-6 PMID: 15625872
  58. Structure of ribulose-1,5-bisphosphate carboxylase-oxygenase: Form III crystals.
    Proc Natl Acad Sci U S A. 1977 Mar;74(3):1037-41 PMID: 16592389
  59. Ribulose bisphosphate carboxylase: a two-layered, square-shaped molecule of symmetry 422.
    Science. 1977 Apr 15;196(4287):293-5 PMID: 17756097
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.137.1.490-501.1979
Published
1979-01-00
Pages
490-501
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC218475
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.137.1.490-501.1979 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2