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PMID: 3317413 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Determinants of membrane protein topology.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 23 ·1987-12-00 ·Pages 8525-9

Boyd D, Manoil C, Beckwith J

Abstract

The topology of the integral membrane protein MalF, which is required for maltose transport in Escherichia coli, has been analyzed using fusions of alkaline phosphatase (EC 3.1.3.1). The properties of such fusion strains support a MalF structure previously proposed on theoretical grounds. Several transmembrane segments within MalF can act as signal sequences in exporting alkaline phosphatase. Other transmembrane sequences, in conjunction with cytoplasmic domains, can stably anchor alkaline phosphatase in the cytoplasm. Our results suggest that features of the amino acid sequence (possibly the positively charged amino acids) of the cytoplasmic domains of membrane proteins are important in anchoring these domains in the cytoplasm. These studies in conjunction with our earlier results show that alkaline phosphatase fusions to membrane proteins can be an important aid in analyzing membrane topology and its determinants.

MeSH Terms

ATP-Binding Cassette Transporters Alkaline Phosphatase Carrier Proteins DNA Mutational Analysis Escherichia coli Escherichia coli Proteins Maltose-Binding Proteins Membrane Proteins Monosaccharide Transport Proteins Protein Conformation Recombinant Fusion Proteins Recombinant Proteins Structure-Activity Relationship

Chemicals

ATP-Binding Cassette Transporters Carrier Proteins Escherichia coli Proteins Maltose-Binding Proteins Membrane Proteins Monosaccharide Transport Proteins Recombinant Fusion Proteins Recombinant Proteins maltose transport system, E coli Alkaline Phosphatase

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Boyd D

Department of Microbiology and Molecular Biology, Harvard Medical School, Boston, MA 02115.

Manoil C

Beckwith J

References (22)

22 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.84.23.8525

Published

1987-12-00

Pages

8525-9

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC299577

Subset

External Links

PubMed source DOI: 10.1073/pnas.84.23.8525 PMC Full Text View journal details

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