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PMID: 3320051 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Processing of a wheat light-harvesting chlorophyll a/b protein precursor by a soluble enzyme from higher plant chloroplasts.

The Journal of cell biology ·Vol. 105 ·No. 6 Pt 1 ·1987-12-00 ·Pages 2641-8

Lamppa GK, Abad MS

Abstract

A processing activity has been identified in higher plant chloroplasts that cleaves the precursor of the light-harvesting chlorophyll a/b-binding protein (LHCP). A wheat LHCP gene previously characterized (Lamppa, G.K., G. Morelli, and N.-H. Chua, 1985. Mol. Cell Biol. 5:1370-1378) was used to synthesize RNA and subsequently the labeled precursor polypeptide in vitro. Incubation of the LHCP precursors with a soluble extract from lysed chloroplasts, after removal of the thylakoids and membrane vesicles, resulted in the release of a single 25-kD peptide. In contrast, when the LHCP precursors were used in an import reaction with intact pea or wheat chloroplasts, two forms (25 and 26 kD) of mature LHCP were found. The peptide released by the processing activity in the organelle-free assay comigrated with the lower molecular mass form of mature LHCP produced during import. Properties of the processing activity suggest that it is an endopeptidase. Chloroplasts from both pea and wheat, two divergent higher plants, contain the processing enzyme, suggesting its physiological importance in LHCP assembly into the thylakoids. We discuss the implications of LHCP precursor processing by a soluble enzyme that may be in the stromal compartment.

MeSH Terms
Amino Acid Sequence Chlorophyll/genetics Chloroplasts/enzymology Genes Light-Harvesting Protein Complexes Photosynthetic Reaction Center Complex Proteins Plant Proteins/genetics Plants/genetics,metabolism Plasmids Protein Precursors/genetics Protein Processing, Post-Translational Triticum/genetics,metabolism
Chemicals
Light-Harvesting Protein Complexes Photosynthetic Reaction Center Complex Proteins Plant Proteins Protein Precursors Chlorophyll
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lamppa G K
Department of Molecular Genetics and Cell Biology, University of Chicago, Illinois 60637.
Abad M S
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.105.6.2641
Published
1987-12-00
Pages
2641-8
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2114706
Subset
IM
Grants
NIGMS NIH HHS · GM36419-02 · United States
External Links
PubMed source DOI: 10.1083/jcb.105.6.2641 PMC Full Text View journal details
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