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PMID: 3321060 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Human immunodeficiency virus protease expressed in Escherichia coli exhibits autoprocessing and specific maturation of the gag precursor.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 24 ·1987-12-00 ·Pages 8903-6

Debouck C, Gorniak JG, Strickler JE, Meek TD, Metcalf BW, Rosenberg M

Abstract

The mature gag and pol proteins of human immunodeficiency virus (HIV) and all retroviruses derive from large gag and gag-pol polyprotein precursors by posttranslational cleavage. A highly specific, virally encoded protease is required for this essential proteolytic processing. In this study, the HIV protease gene product was expressed in Escherichia coli and shown to autocatalyze its maturation from a larger precursor. In addition, this bacterially produced HIV protease specifically processed an HIV p55 gag polyprotein precursor when coexpressed in E. coli. This system will allow detailed structure-function analysis of the HIV protease and provides a simple assay for the development of potential therapeutic agents directed against this critical viral enzyme.

MeSH Terms

Amino Acid Sequence Cloning, Molecular Endopeptidases/genetics Escherichia coli/metabolism Gene Products, gag Genes, Viral HIV/enzymology,genetics HIV Protease Molecular Weight Protein Processing, Post-Translational Recombinant Proteins Retroviridae Proteins/metabolism

Chemicals

Gene Products, gag Recombinant Proteins Retroviridae Proteins Endopeptidases HIV Protease

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Debouck C

Department of Molecular Genetics, Smith Kline and French Laboratories, King of Prussia, PA 19406.

Gorniak J G

Strickler J E

Meek T D

Metcalf B W

Rosenberg M

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.84.24.8903

Published

1987-12-00

Pages

8903-6

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC299659

Subset

Grants

NIAID NIH HHS · AI24845 · United States

External Links

PubMed source DOI: 10.1073/pnas.84.24.8903 PMC Full Text View journal details

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Human immunodeficiency virus protease expressed in Escherichia coli exhibits autoprocessing and specific maturation of the gag precursor.

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