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PMID: 3323844 Published · ppublish English Journal Article

Secretion of Mucor rennin, a fungal aspartic protease of Mucor pusillus, by recombinant yeast cells.

Molecular & general genetics : MGG ·Vol. 210 ·No. 3 ·1987-12-00 ·Pages 462-7

Yamashita T, Tonouchi N, Uozumi T, Beppu T

Abstract

The aspartic protease gene of a zygomycete fungus Mucor pusillus was expressed in Saccharomyces cerevisiae under the control of the yeast GAL7 promoter. A putative preproenzyme with an NH2-terminal extension of 66 amino acids directed by the gene was processed in yeast cells and the mature enzyme, whose NH2-terminus was identical to that of the Mucor enzyme, was efficiently secreted into the medium at a concentration exceeding 150 mg/l. The enzyme secreted from the recombinant yeast was more glycosylated than the native Mucor enzyme but its enzymatic properties were almost identical with those of the native enzyme, which has been used as a milk coagulant in cheese manufacture.

MeSH Terms
Amino Acid Sequence Animals Aspartic Acid Endopeptidases Calcium Cloning, Molecular Endopeptidases/genetics,metabolism Gene Expression Regulation Genes, Fungal Glycosylation Milk Molecular Sequence Data Mucor/enzymology,genetics Plasmids Protein Processing, Post-Translational Recombinant Proteins/metabolism Saccharomyces cerevisiae/genetics
Chemicals
Recombinant Proteins Endopeptidases Aspartic Acid Endopeptidases rennin-like enzyme (Aspergillus ochraceus) Calcium
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Yamashita T
Department of Agricultural Chemistry, Faculty of Agriculture, University of Tokyo, Japan.
Tonouchi N
Uozumi T
Beppu T
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19 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
Published
1987-12-00
Pages
462-7
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
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