Home LiteratureArticle Details
PMID: 333395 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

RNA-ligant interactions. (I) Magnesium binding sites in yeast tRNAPhe.

Nucleic acids research ·Vol. 4 ·No. 8 ·1977-08-00 ·Pages 2811-20

Holbrook SR, Sussman JL, Warrant RW, Church GM, Kim SH

Abstract

X-ray crystallagraphic studies studies indicate that there are at least four site-specifically bound hydrated Mg2+ ions, [Mg(H2O)n]2+, in yeast tRNAPhe. The size and the octahedral coordination geometry, rather than the charge, of [Mg(H2O)N]2+ appear to be the primary reasons for the specificity of magnesium ions in site-binding and in the stabilization of the tertiary structure of tRNA.

MeSH Terms
Binding Sites Chemical Phenomena Chemistry Ligands Magnesium Models, Molecular Nucleic Acid Conformation Phenylalanine RNA, Transfer Saccharomyces cerevisiae X-Ray Diffraction
Chemicals
Ligands Phenylalanine RNA, Transfer Magnesium
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Holbrook S R
Sussman J L
Warrant R W
Church G M
Kim S H
References (19)
19 references, click to expand
  1. A crystallographic study of metal-binding to yeast phenylalanine transfer RNA.
    J Mol Biol. 1977 Apr 15;111(3):315-28 PMID: 325214
  2. High-resolution x-ray diffraction patterns of crystalline transfer RNA that show helical regions.
    Proc Natl Acad Sci U S A. 1971 Apr;68(4):841-5 PMID: 5279525
  3. tRNA structure and binding sites for cations.
    Biopolymers. 1972;11(7):1317-33 PMID: 4557654
  4. Investigation of the structure of yeast tRNAphe by nuclear magnetic resonance: paramagnetic rare earth ion probes of structure.
    Proc Natl Acad Sci U S A. 1974 Oct;71(10):4237-40 PMID: 4610573
  5. Enhancement of Tb(III) and Eu(III) fluorescence in complexes with Escherichia coli tRNA.
    Biochemistry. 1974 Sep 24;13(20):4159-65 PMID: 4606518
  6. Cation requirements of isoleucyl-tRNA synthetase from Escherichia coli.
    Biochem Biophys Res Commun. 1972 Feb 16;46(3):1285-91 PMID: 4334974
  7. Proton magnetic relaxation studies of marganous complexes of transfer RNA and related compounds.
    J Mol Biol. 1969 Jan 14;39(1):199-217 PMID: 4329286
  8. Cooperative binding of manganese (II) to transfer RNA.
    Eur J Biochem. 1970 Nov;16(3):532-6 PMID: 4320087
  9. Thermodynamic studies of transfer ribonucleic acids. I. Magnesium binding to yeast phenylalanine transfer ribonucleic acid.
    Biochemistry. 1972 Jun 20;11(13):2472-9 PMID: 4625190
  10. Interaction of manganese with fragments, complementary fragment recombinations, and whole molecules of yeast phenylalanine specific transfer RNA.
    J Mol Biol. 1974 Jul 5;86(3):601-20 PMID: 4604622
  11. Three-dimensional structure of yeast phenylalanine transfer RNA at 3.0angstroms resolution.
    Nature. 1974 Mar 1;248(5443):20-4 PMID: 4594440
  12. A new method for specific labelling of tRNA: preliminary results on yeast tRNA Phe .
    Biochimie. 1972;54(3):333-7 PMID: 4565464
  13. Three-dimensional tertiary structure of yeast phenylalanine transfer RNA.
    Science. 1974 Aug 2;185(4149):435-40 PMID: 4601792
  14. Renaturation of transfer ribonucleic acids through site binding of magnesium.
    Proc Natl Acad Sci U S A. 1966 Apr;55(4):941-8 PMID: 5327073
  15. Europium as a fluorescent probe of transfer RNA structure.
    Biochemistry. 1975 Apr 8;14(7):1436-44 PMID: 1092336
  16. tRNA conformation and magnesium binding. A study of a yeast phenylalanine-specific tRNA by a fluorescent indicator and differential melting curves.
    Eur J Biochem. 1975 Jun 16;55(1):271-84 PMID: 1100382
  17. Equilibrium binding of magnesium(II) by Escherichia coli tRNAfMet.
    Biochemistry. 1976 Jan 13;15(1):157-60 PMID: 764857
  18. Idealized atomic coordinates of yeast phenylalanine transfer RNA.
    Biochem Biophys Res Commun. 1976 Jan 12;68(1):89-96 PMID: 1108880
  19. Tertiary structure in transfer ribonucleic acids.
    Cold Spring Harb Symp Quant Biol. 1966;31:527-37 PMID: 5237202
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/4.8.2811
Published
1977-08-00
Pages
2811-20
Language
English
Region
England
NLM ID
0411011
PMCID
PMC342610
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/4.8.2811 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2