Excision of F plasmid sequences by recombination at directly repeated insertion sequence 2 elements: involvement of recA.
The DNA of the F plasmid is joined to bacterial DNA sequences in the F' ORF203 by directly repeated insertion sequence 2 (IS2) elements. The rate of excision of the F plasmid form this F' (presumably by recombination at the directly repeated IS2s) has been estimated in both recA+ and recA- strains. Normal F is produced in the recA+ strain, but is not detected in recA-. The autonomous plasmids produced in the recA- background were F's having deletions. F excision in this particular recA+ case is specific in the sense that the directly repeated IS2s appear to be more active in recombination than similarly disposed IS3 direct repetitions in this F'.
19 references, click to expand
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