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PMID: 3336365 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The cap of both miniexon-derived RNA and mRNA of trypanosomes is 7-methylguanosine.

Molecular and cellular biology ·Vol. 8 ·No. 1 ·1988-01-00 ·Pages 494-6

Sutton RE, Boothroyd JC

Abstract

Most, if not all, trypanosome mRNAs have the same 35-base sequence at their 5' terminus which is derived from a short RNA (medRNA) probably by the process of trans-splicing. It is of interest, evolutionarily and mechanistically, to determine the chemical structure of the 5' terminus of the precursor (medRNA) and product (mRNA). We demonstrate here that the cap structure of both is most probably 7-methylguanosine in a 5',5' triphosphate linkage, consistent with a precursor/product relationship.

MeSH Terms

Animals Exons Guanosine/analogs & derivatives,genetics RNA Caps RNA Splicing RNA, Messenger/genetics Trypanosoma brucei brucei/genetics

Chemicals

RNA Caps RNA, Messenger Guanosine 7-methylguanosine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Sutton R E

Department of Medical Microbiology, Stanford University School of Medicine, California 94305.

Boothroyd J C

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27 references, click to expand

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Article Info

Journal

Molecular and cellular biology

Abbr.

Mol Cell Biol

ISSN

0270-7306

DOI

10.1128/mcb.8.1.494-496.1988

Published

1988-01-00

Pages

494-6

Language

English

Region

United States

NLM ID

8109087

PMCID

PMC363158

Subset

Grants

NIAID NIH HHS · AI21023 · United States

NIGMS NIH HHS · GM07365 · United States

External Links

PubMed source DOI: 10.1128/mcb.8.1.494-496.1988 PMC Full Text View journal details

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The cap of both miniexon-derived RNA and mRNA of trypanosomes is 7-methylguanosine.

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