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PMID: 3344202 Published · ppublish English Comparative Study Journal Article

Sequence of cDNAs for mammalian H2A.Z, an evolutionarily diverged but highly conserved basal histone H2A isoprotein species.

Nucleic acids research ·Vol. 16 ·No. 3 ·1988-02-11 ·Pages 1113-24

Hatch CL, Bonner WM

Abstract

The nucleotide sequences of cDNAs for the evolutionarily diverged but highly conserved basal H2A isoprotein, H2A.Z, have been determined for the rat, cow, and human. As a basal histone, H2A.Z is synthesized throughout the cell cycle at a constant rate, unlinked to DNA replication, and at a much lower rate in quiescent cells. Each of the cDNA isolates encodes the entire H2A.Z polypeptide. The human isolate is about 1.0 kilobases long. It contains a coding region of 387 nucleotides flanked by 106 nucleotides of 5'UTR and 376 nucleotides of 3'UTR, which contains a polyadenylation signal followed by a poly A tail. The bovine and rat cDNAs have 97 and 94% nucleotide positional identity to the human cDNA in the coding region and 98% in the proximal 376 nucleotides of the 3'UTR which includes the polyadenylation signal. A potential stem-forming sequence imbedded in a direct repeat is found centered at 261 nucleotides into the 3'UTR. Each of the cDNA clones could be transcribed and translated in vitro to yield H2A.Z protein. The mammalian H2A.Z cDNA coding sequences are approximately 80% similar to those in chicken and 75% to those in sea urchin.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cattle/genetics Chickens/genetics DNA/genetics Histones/genetics Humans Molecular Sequence Data Rats/genetics Recombinant Proteins/biosynthesis,genetics Sea Urchins/genetics Sequence Homology, Nucleic Acid Species Specificity
Chemicals
Histones Recombinant Proteins DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hatch C L
Laboratory of Molecular Pharmacology, National Cancer Institute, Bethesda, MD 20892.
Bonner W M
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/16.3.1113
Published
1988-02-11
Pages
1113-24
Language
English
Region
England
NLM ID
0411011
PMCID
PMC334740
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/16.3.1113 PMC Full Text View journal details
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