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PMID: 334724 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Tryptophan-transducing bacteriophages: in vitro studies with restriction endonucleases HindII + III and Escherichia coli ribonucleic acid polymerase.

Journal of bacteriology ·Vol. 132 ·No. 1 ·1977-10-00 ·Pages 270-81

Jones BB, Reznikoff WS

Abstract

The HindII + III restriction enzyme fragmentation pattern of various lambda-phi80trp deoxyribonucleic acid molecules is presented. An analysis of deoxyribonucleic acid molecules carrying deletions ending within the trp regulatory elements and a deoxyribonucleic acid molecule carrying a deletion within trpE indicates that a fragment of 8.3 X 10(5) daltons contains at least part of the trp promoter, the entire trp leader region, and part of the trpE gene. The observation that ribonucleic acid polymerase, when present in the HindII + III digestion mixture, results in the fusion of this 8.3 X 10(5)-dalton fragment to the preceding bacterial fragment suggests that HindII + III cuts within trpP.

MeSH Terms
Coliphages/analysis,genetics,metabolism DNA Restriction Enzymes/metabolism DNA, Viral/genetics,metabolism DNA-Directed RNA Polymerases/metabolism Escherichia coli/enzymology Molecular Weight Operon Protein Binding Transduction, Genetic Tryptophan/biosynthesis,genetics
Chemicals
DNA, Viral Tryptophan DNA-Directed RNA Polymerases DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Jones B B
Reznikoff W S
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24 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.132.1.270-281.1977
Published
1977-10-00
Pages
270-81
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC221853
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.132.1.270-281.1977 PMC Full Text View journal details
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