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PMID: 3348359 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Isolation of a major protein component of Rosenthal fibers.

The American journal of pathology ·Vol. 130 ·No. 3 ·1988-03-00 ·Pages 569-78

Goldman JE, Corbin E

Abstract

Rosenthal fibers (RFs) are inclusions within astrocytes, characteristic of Alexander's disease, but also seen in astrocytic tumors and occasionally in glial scar tissue. They are granular deposits, intimately associated with intermediate filaments. Their composition has been unknown. The authors have isolated a protein of about 19 kd from partially purified RFs from Alexander's disease central nervous system tissue. Antibodies were raised to this protein and shown to react with it on nitrocellulose blots and to bind to RFs in tissue sections. Small amounts of this protein were detected in normal brain and in cultured rat astrocytes. Charge heterogeneity was inferred because several species were separated by isoelectric focusing.

MeSH Terms
Animals Antibodies Astrocytes/analysis,ultrastructure Brain Chemistry Brain Diseases/metabolism,pathology Cell Fractionation Cells, Cultured Electrophoresis, Polyacrylamide Gel Glial Fibrillary Acidic Protein/analysis Humans Immunohistochemistry Intermediate Filament Proteins/isolation & purification Isoelectric Focusing Microscopy, Electron Nerve Tissue Proteins/isolation & purification Rats Tissue Extracts/analysis Vimentin/analysis
Chemicals
Antibodies Glial Fibrillary Acidic Protein Intermediate Filament Proteins Nerve Tissue Proteins Tissue Extracts Vimentin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Goldman J E
Department of Pathology (Neuropathology), Albert Einstein College of Medicine, Bronx, New York.
Corbin E
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Article Info
Journal
The American journal of pathology
Abbr.
Am J Pathol
ISSN
0002-9440
Published
1988-03-00
Pages
569-78
Language
English
Region
United States
NLM ID
0370502
PMCID
PMC1880665
Subset
IM
Grants
NINDS NIH HHS · NS17125 · United States
External Links
PubMed source PMC Full Text View journal details
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