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PMID: 3356459 Published · ppublish English Comparative Study Journal Article

Identification of the major antigens of Treponema hyodysenteriae and comparison with those of Treponema innocens.

Infection and immunity ·Vol. 56 ·No. 5 ·1988-05-00 ·Pages 1070-5

Chatfield SN, Fernie DS, Penn C, Dougan G

Abstract

Eleven strains of Treponema hyodysenteriae isolated from pigs with swine dysentery were examined by using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting. T. hyodysenteriae strains formed a homogeneous group with respect to sodium dodecyl sulfate-soluble proteins. However, immunoblotting with antiserum from rabbits immunized with T. hyodysenteriae CN8368 revealed heterogeneity among the lipopolysaccharide complexes of different strains. Polypeptides of molecular weights between 30,000 and 36,000 were the predominant T. hyodysenteriae polypeptides detected by porcine immune serum. In contrast, Treponema innocens did not form a homogeneous group with respect to sodium dodecyl sulfate-soluble proteins. Adsorption studies and immunoblotting identified polypeptide antigens present on cells of T. hyodysenteriae which were not detected on cells of T. innocens. These unique antigens may play a role in the virulence of T. hyodysenteriae.

MeSH Terms
Animals Antigens, Bacterial/analysis Cell Fractionation Dysentery/microbiology Electrophoresis, Polyacrylamide Gel Molecular Weight Solubility Swine Swine Diseases/microbiology Treponema/immunology Treponemal Infections/immunology
Chemicals
Antigens, Bacterial
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Chatfield S N
Department of Molecular Biology, Wellcome Research Laboratories, Beckenham, Kent, United Kingdom.
Fernie D S
Penn C
Dougan G
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.56.5.1070-1075.1988
Published
1988-05-00
Pages
1070-5
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC259764
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.56.5.1070-1075.1988 PMC Full Text View journal details
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