A stable complex between homopyrimidine oligomers and the homologous regions of duplex DNAs.

Nucleic acids research ·Vol. 16 ·No. 5 ·1988-03-25 ·Pages 2165-78

Lyamichev VI, Mirkin SM, Frank-Kamenetskii MD, Cantor CR

Abstract

When plasmid DNA duplexes carrying the regular homopurine-homopyrimidine inserts (dGA)n, (dTC)n and (dG)n, (dC)n are preincubated with homologous labeled oligo(dPy) ((dTC)n and (dC)n respectively) at acid pH, the label co-electrophoreses with the duplex DNA. Thus, a very strong complex is formed. Complementary oligo(dPu) does not form a complex under these conditions. No binding is observed for oligo(dPy) with non-homologous inserts as well as with vector plasmids without inserts. The complex is formed equally well with linear, nicked or superhelical DNA. The complex is not detected at pH greater than 6. Complex formation leads to very little, if any, unwinding of the duplex. The observed complex appears to be the Py.Pu.Py triplex consisting of TAT and CGC base-triads with protonated cytosines. Two-dimensional gel electrophoresis patterns show that the presence of homologous oligo(dPy) destabilizes the formation of the H form.

MeSH Terms

DNA DNA, Superhelical Electrophoresis, Agar Gel Macromolecular Substances Nucleic Acid Conformation Oligodeoxyribonucleotides Pyrimidines

Chemicals

DNA, Superhelical Macromolecular Substances Oligodeoxyribonucleotides Pyrimidines DNA

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Lyamichev V I

Institute of Molecular Genetics, USSR Academy of Sciences, Moscow.

Mirkin S M

Frank-Kamenetskii M D

Cantor C R

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/16.5.2165

Published

1988-03-25

Pages

2165-78

Language

English

Region

England

NLM ID

0411011

PMCID

PMC338206

Subset

External Links

PubMed source DOI: 10.1093/nar/16.5.2165 PMC Full Text View journal details

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A stable complex between homopyrimidine oligomers and the homologous regions of duplex DNAs.

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