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PMID: 337108 Published · ppublish English Journal Article

Prophage induction in Escherichia coli K12 cells deficient in DNA polymerase I.

Molecular & general genetics : MGG ·Vol. 154 ·No. 3 ·1977-09-09 ·Pages 287-92

Blanco M, Pomes L

Abstract

The induction of prophage lambda by ultraviolet light has been measured in E. coli K12 lysogenic cells deficient in DNA polymerase I. The efficiency of the induction process was greater in polA1 polC(dnaE) double mutants incubated at the temperature that blocks DNA replication than in polA+ polC single mutants. Similarly, the polA1 mutation sensitized tif-promoted lysogenic induction in a polA1 tif strain at 42 degrees. In strains bearing the polA12 mutation, which growth normally at 30 degrees, induction of the prophage occurred after the shift to 42 degrees. It is concluded that dissapearance of the DNA polymerase I activity leads to changes in DNA replication that are able, per se, trigger the prophage induction process.

MeSH Terms
Coliphages DNA Polymerase I/metabolism DNA, Bacterial/metabolism DNA-Directed DNA Polymerase/metabolism Escherichia coli/enzymology Lysogeny/radiation effects Mutation Ultraviolet Rays
Chemicals
DNA, Bacterial DNA Polymerase I DNA-Directed DNA Polymerase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Blanco M
Pomes L
References (23)
23 references, click to expand
  1. Inviability of recA- derivatives of the DNA polymerase mutant of De Lucia and Cairns.
    J Mol Biol. 1971 Jun 14;58(2):631-4 PMID: 4933421
  2. Temperature-sensitive regulation system of prophage lambda induction.
    J Mol Biol. 1967 Jan 28;23(2):217-24 PMID: 5340185
  3. DNA polymerase 3 star requires ATP to start synthesis on a primed DNA.
    Proc Natl Acad Sci U S A. 1973 Dec;70(12):3679-83 PMID: 4519657
  4. Model for regulation of Escherichia coli DNA repair functions.
    Proc Natl Acad Sci U S A. 1975 Jun;72(6):2330-4 PMID: 1094463
  5. A DNA-unwinding protein isolated from Escherichia coli: its interaction with DNA and with DNA polymerases.
    Proc Natl Acad Sci U S A. 1972 Dec;69(12):3537-41 PMID: 4566449
  6. DNA polymerase I: essential replication enzyme.
    Science. 1976 Sep 10;193(4257):963-9 PMID: 781842
  7. Lysogenic induction.
    Prog Nucleic Acid Res Mol Biol. 1973;13:249-300 PMID: 4267058
  8. Biochemical characterization of mutant forms of DNA polymerase I from Escherichia coli. I. The polA12 mutation.
    J Biol Chem. 1976 Jul 10;251(13):4078-84 PMID: 6470
  9. Roles for E. coli DNA polymerases I, II, and 3 in DNA replication.
    Nature. 1974 May 10;249(453):116-9 PMID: 4598693
  10. Prophage induction and filament formation in a mutant strain of Escherichia coli.
    Proc Natl Acad Sci U S A. 1967 Nov;58(5):1903-10 PMID: 4866981
  11. IexB: a new gene governing radiation sensitivity and lysogenic induction in Escherichia coli K12.
    Basic Life Sci. 1975;5A:379-82 PMID: 1103847
  12. Stimulation of the synthesis of bacteriophage T4 gene 32 protein by ultraviolet light irradiation.
    J Mol Biol. 1976 Nov;108(1):67-81 PMID: 1003487
  13. Prophage induction and cell division in E. coli. I. Further characterization of the thermosensitive mutation tif-1 whose expression mimics the effect of UV irradiation.
    Mol Gen Genet. 1972;119(2):139-52 PMID: 4565754
  14. Repair of damage induced by ultraviolet light in DNA polymerase-defective Escherichia coli cells.
    J Mol Biol. 1971 Jun 14;58(2):623-30 PMID: 4933420
  15. Conditional lethality of recA and recB derivatives of a strain of Escherichia coli K-12 with a temperature-sensitive deoxyribonucleic acid polymerase I.
    J Bacteriol. 1972 Mar;109(3):971-8 PMID: 4551758
  16. Prophage lambda induction of Escherichia coli K12 envA uvrB: a highly sensitive test for potential carcinogens.
    Proc Natl Acad Sci U S A. 1976 Oct;73(10):3700-4 PMID: 790393
  17. Regulation of gene 32 expression during bacteriophage T4 infection of Escherichia coli.
    J Biol Chem. 1976 Nov 25;251(22):7251-62 PMID: 791947
  18. Recalibrated linkage map of Escherichia coli K-12.
    Bacteriol Rev. 1976 Mar;40(1):116-67 PMID: 773363
  19. Slow joining of newly replicated DNA chains in DNA polymerase I-deficient Escherichia coli mutants.
    Proc Natl Acad Sci U S A. 1971 Dec;68(12):2954-7 PMID: 4943548
  20. Prophage induction by high temperature in thermosensitive dna mutants lysogenic for bacteriophage lambda.
    J Virol. 1973 Jun;11(6):879-85 PMID: 4576518
  21. Genetics and function of DNA ligase in Escherichia coli.
    J Mol Biol. 1973 Jul 15;77(4):531-47 PMID: 4353284
  22. Ultraviolet mutagenesis in polA and UvrA polA derivatives of Escherichia coli B-R: evidence for an inducible error-prone repair system.
    Genetics. 1973 Apr;73:Suppl 73:91-10 PMID: 4576123
  23. A method for the isolation of phage mutants altered in their response ot lysogenic induction.
    Mol Gen Genet. 1976 May 7;145(2):165-7 PMID: 934047
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00571284
Published
1977-09-09
Pages
287-92
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00571284
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