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PMID: 3377754 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Isolation and biochemical analysis of ethyl methanesulfonate-induced alcohol dehydrogenase null mutants of arabidopsis thaliana (L.) Heynh.

Biochemical genetics ·Vol. 26 ·No. 1-2 ·1988-02-00 ·Pages 105-22

Jacobs M, Dolferus R, Van den Bossche D

Abstract

Several mutants have been isolated at the Arabidopsis thaliana (L.) Heynh. alcohol dehydrogenase (ADH) gene locus using allyl alcohol selection on ethyl methanesulfonate (EMS)-mutagenized seeds. Eleven mutants were isolated in the ADH1-A electrophoretic allele, and 21 in the ADH1-S allele. These null mutants are characterized by the absence of measurable ADH activity and genetic data showed that the mutations were confined to the ADH1 gene locus of Arabidopsis. Eleven mutants in the ADH1-A background were further characterized at the protein and mRNA level. These experiments revealed striking differences in the ADH protein and mRNA content. Some of the mutants did not synthesize any mRNA or ADH-like protein, whereas some of them had a nearly normal level of ADH protein and mRNA. Others had a very low level of both protein and mRNA. ADH null mutants differed physiologically from the wild type by their higher sensitivity to anaerobic treatment in plants and significantly reduced resistance to acetaldehyde in suspension cultures.

MeSH Terms

1-Propanol/pharmacology Alcohol Dehydrogenase/genetics Crosses, Genetic Ethyl Methanesulfonate/pharmacology Genes Mutation Nucleic Acid Hybridization Plants/drug effects,enzymology,genetics Propanols RNA, Messenger/genetics

Chemicals

Propanols RNA, Messenger allyl alcohol 1-Propanol Ethyl Methanesulfonate Alcohol Dehydrogenase

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Jacobs M

Laboratory of Plant Genetics, Free University of Brussels, Belgium.

Dolferus R

Van den Bossche D

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23 references, click to expand

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Article Info

Journal

Biochemical genetics

Abbr.

Biochem Genet

ISSN

0006-2928

DOI

10.1007/BF00555492

Published

1988-02-00

Pages

105-22

Language

English

Region

United States

NLM ID

0126611

Subset

External Links

PubMed source DOI: 10.1007/BF00555492 View journal details

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Isolation and biochemical analysis of ethyl methanesulfonate-induced alcohol dehydrogenase null mutants of arabidopsis thaliana (L.) Heynh.

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