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PMID: 3380090 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

RAP30/74: a general initiation factor that binds to RNA polymerase II.

Molecular and cellular biology ·Vol. 8 ·No. 4 ·1988-04-00 ·Pages 1602-13

Burton ZF, Killeen M, Sopta M, Ortolan LG, Greenblatt J

Abstract

We have previously shown by affinity chromatography that RAP30 and RAP74 are the mammalian proteins that have the highest affinity for RNA polymerase II. Here we show that RAP30 binds to RAP74 and that the RAP30-RAP74 complex (RAP30/74) is required for accurate initiation by RNA polymerase II. RAP30/74 is required for accurate transcription from the following promoters: the adenovirus major late promoter, the long terminal repeat of human immunodeficiency virus, P2 of the human c-myc gene, the mouse beta maj-globin promoter (all of which have TATA boxes), and the mouse dihydrofolate reductase promoter (which lacks a TATA box). RAP30/74 is not required for initiation by RNA polymerase III at the adenovirus virus-associated RNA promoters. Therefore, RAP30/74 is a general initiation factor that binds to RNA polymerase II.

MeSH Terms
Carrier Proteins/isolation & purification,metabolism Genes Genes, Viral HeLa Cells/enzymology Humans Kinetics Phosphoproteins/isolation & purification,metabolism Promoter Regions, Genetic Protein Binding Proto-Oncogenes RNA Polymerase II/metabolism Transcription, Genetic
Chemicals
Carrier Proteins Phosphoproteins RNA polymerase II-binding proteins RNA Polymerase II
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Burton Z F
Banting and Best Department of Medical Research, University of Toronto, Ontario, Canada.
Killeen M
Sopta M
Ortolan L G
Greenblatt J
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.4.1602-1613.1988
Published
1988-04-00
Pages
1602-13
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC363320
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.8.4.1602-1613.1988 PMC Full Text View journal details
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