Arrangement of protein I in Escherichia coli outer membrane: cross-linking study.

Journal of bacteriology ·Vol. 133 ·No. 1 ·1978-01-00 ·Pages 279-86

Palva ET, Randall LL

Abstract

The arrangement of protein I in the outer membrane of Escherichia coli was investigated by cross-linking whole cells, isolated cell wall, protein-peptidoglycan complexes, and protein I released from peptidoglycan with NaCl. Both cleavable azide cross-linkers and imidoester reagents were used. The data presented suggest that protein I exists in the outer membrane as a trimer.

MeSH Terms

Azides Bacterial Proteins Cell Wall Dimethyl Suberimidate Electrophoresis, Polyacrylamide Gel Escherichia coli/ultrastructure Membrane Proteins Molecular Weight Peptidoglycan Protein Conformation

Chemicals

Azides Bacterial Proteins Membrane Proteins Peptidoglycan Dimethyl Suberimidate

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Palva E T

Randall L L

References (24)

24 references, click to expand

Interactions of outer membrane proteins O-8 and O-9 with peptidoglycan sacculus of Escherichia coli K-12.
J Biochem. 1976 Dec;80(6):1401-9 PMID: 14126
Ultrastructure of a periodic protein layer in the outer membrane of Escherichia coli.
J Cell Biol. 1977 Feb;72(2):292-301 PMID: 319099
Stimulation by lipopolysaccharide of the binding of outer membrane proteins O-8 and O-9 to the peptidoglycan layer of Escherichia coli K--12.
Biochem Biophys Res Commun. 1977 Feb 21;74(4):1397-402 PMID: 320978
Cross-linking of the proteins in the outer membrane of Escherichia coli.
Biochim Biophys Acta. 1977 Apr 18;466(2):245-56 PMID: 322714
The major proteins of the Escherichia coli outer cell-envelope membrane. Heterogeneity of protein I.
Eur J Biochem. 1976 Mar 16;63(1):47-52 PMID: 770169
Nearest-neighbor analysis of Escherichia coli outer membrane proteins using cleavable cross-links.
J Bacteriol. 1976 Sep;127(3):1558-60 PMID: 783148
Identification of the outer membrane protein of E. coli that produces transmembrane channels in reconstituted vesicle membranes.
Biochem Biophys Res Commun. 1976 Aug 9;71(3):877-84 PMID: 786294
Outer membrane of Salmonella XIV. Reduced transmembrane diffusion rates in porin-deficient mutants.
Biochem Biophys Res Commun. 1976 May 23;76(2):324-30 PMID: 800339
Outer membrane as a diffusion barrier in Salmonella typhimurium. Penetration of oligo- and polysaccharides into isolated outer membrane vesicles and cells with degraded peptidoglycan layer.
J Biol Chem. 1975 Sep 25;250(18):7359-65 PMID: 1100625
Repetitive sequences in the murein-lipoprotein of the cell wall of Escherichia coli.
Proc Natl Acad Sci U S A. 1972 Apr;69(4):970-4 PMID: 4260278
Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane.
Biochemistry. 1971 Jun 22;10(13):2606-17 PMID: 4326772
The use of a new series of cleavable protein-crosslinkers on the Escherichia coli ribosome.
FEBS Lett. 1974 Nov 15;48(2):288-92 PMID: 4373293
Mechanism of assembly of the outer membrane of Salmonella typhimurium. Isolation and characterization of cytoplasmic and outer membrane.
J Biol Chem. 1972 Jun 25;247(12):3962-72 PMID: 4555955
Measurement of free electrophoretic mobility and retardation coefficient of protein-sodium dodecyl sulfate complexes by gel electrophoresis. A method to validate molecular weight estimates.
J Biol Chem. 1972 Sep 25;247(18):5856-61 PMID: 4560419
Cell envelope and shape of Escherichia coli K12.
Proc Natl Acad Sci U S A. 1973 Jul;70(7):2033-6 PMID: 4579010
Cell envelope and shape of Escherichia coli K12. The ghost membrane.
Eur J Biochem. 1973 Nov 1;39(1):27-36 PMID: 4589028
Mutants of Escherichia coli defective in membrane phospholipid synthesis: macromolecular synthesis in an sn-glycerol 3-phosphate acyltransferase Km mutant.
J Bacteriol. 1974 Mar;117(3):1065-76 PMID: 4591941
Cell envelope and shape of Escherichia coli K12. Crosslinking with dimethyl imidoesters of the whole cell wall.
Proc Natl Acad Sci U S A. 1974 May;71(5):2018-21 PMID: 4599990
Cell envelope and shape of Escherichia coli K12. Isolation and preliminary characterization of the major ghost-membrane proteins.
Eur J Biochem. 1974 Sep 1;47(2):343-52 PMID: 4606317
Characterization of the major envelope protein from Escherichia coli. Regular arrangement on the peptidoglycan and unusual dodecyl sulfate binding.
J Biol Chem. 1974 Dec 25;249(24):8019-29 PMID: 4609976
[Phenotypic expression and genetic localization of mutations affecting maltose metabolism in Escherichia coli K 12].
Ann Inst Pasteur (Paris). 1967 Jun;112(6):673-98 PMID: 4862550
Use of dimethyl suberimidate, a cross-linking reagent, in studying the subunit structure of oligomeric proteins.
Proc Natl Acad Sci U S A. 1970 Jul;66(3):651-6 PMID: 4913206
Tables for estimating sedimentation through linear concentration gradients of sucrose solution.
Anal Biochem. 1967 Jul;20(1):114-49 PMID: 6034985
Temperate Bacteriophage Which Causes the Production of a New Major Outer Membrane Protein by Escherichia coli.
J Virol. 1975 May;15(5):1121-30 PMID: 16789148

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.133.1.279-286.1978

Published

1978-01-00

Pages

279-86

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC222005

Subset

External Links

PubMed source DOI: 10.1128/jb.133.1.279-286.1978 PMC Full Text View journal details

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Arrangement of protein I in Escherichia coli outer membrane: cross-linking study.

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