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PMID: 338598 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Single-strand breakage in DNA of Escherichia coli exposed to Cd2+.

Journal of bacteriology ·Vol. 133 ·No. 1 ·1978-01-00 ·Pages 75-80

Mitra RS, Bernstein IA

Abstract

When a growing culture of Escherichia coli was exposed to 3 X 10(-6) M Cd2+, 85 to 95% of the cells lost their ability to form colonies on agar plates. Loss of viability was accompanied by considerable single-strand breakage in the DNA, with no detectable increase in double-strand breaks. A direct correlation appeared to exist between the number of single-strand breaks and the concentrations of Cd2+ to which the cells were exposed. Exposure of DNA in vitro to a Cd2+ concentration of 3 X 10(-6) M or higher, followed by sedimentation in alkaline sucrose gradients, demonstrated no single-strand breaks. Cadmium-exposed cells recovered viability when incubated in Cd2+-free liquid medium containing 10 mM hydroxyurea. During the early period of recovery, there was a lag in the incorporation of labeled thymidine, but cellular DNA, at least in part, appeared to be repaired.

MeSH Terms
Cadmium/pharmacology Centrifugation, Density Gradient DNA Repair DNA, Bacterial Escherichia coli/drug effects
Chemicals
DNA, Bacterial Cadmium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mitra R S
Bernstein I A
References (20)
20 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.133.1.75-80.1978
Published
1978-01-00
Pages
75-80
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC221978
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.133.1.75-80.1978 PMC Full Text View journal details
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