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PMID: 3386632 Published · ppublish English Comparative Study Journal Article

Nuclear pre-mRNA processing in plants: distinct modes of 3'-splice-site selection in plants and animals.

Molecular and cellular biology ·Vol. 8 ·No. 5 ·1988-05-00 ·Pages 2042-51

Wiebauer K, Herrero JJ, Filipowicz W

Abstract

The report that human growth hormone pre-mRNA is not processed in transgenic plant tissues (A. Barta, K. Sommergruber, D. Thompson, K. Hartmuth, M.A. Matzke, and A.J.M. Matzke, Plant Mol. Biol. 6:347-357, 1986) has suggested that differences in mRNA splicing processes exist between plants and animals. To gain more information about the specificity of plant pre-mRNA processing, we have compared the splicing of the soybean leghemoglobin pre-mRNA with that of the human beta-globin pre-mRNA in transfected plant (Orychophragmus violaceus and Nicotiana tabacum) protoplasts and mammalian (HeLa) cells. Of the three introns of leghemoglobin pre-mRNA, only intron 2 was correctly and efficiently processed in HeLa cells. The 5' splice sites of the remaining two introns were faithfully recognized, but correct processing of the 3' sites took place only rarely (intron 1) or not at all (intron 3); cryptic 3' splice sites were used instead. While the first intron in human beta-globin pre-mRNA was not spliced in transfected plant protoplasts, intron 2 processing occurred at a low level, indicating that some mammalian introns can be recognized by the plant intron-splicing machinery. However, excision of intron 2 proved to be incorrect, involving the authentic 5' splice site and a cryptic 3' splice site. Our results indicate that the mechanism of 3'-splice-site selection during intron excision differs between plants and animals. This conclusion is supported by analysis of the 3'-splice-site consensus sequences in animal and plant introns which revealed that polypyrimidine tracts, characteristic of animal introns, are not present in plant pre-mRNAs. It is proposed that an elevated AU content of plant introns is important for their processing.

MeSH Terms
Base Sequence HeLa Cells/metabolism Humans Leghemoglobin/genetics Molecular Sequence Data Plants/genetics,metabolism Protoplasts/metabolism RNA Precursors/metabolism RNA Splicing Species Specificity
Chemicals
Leghemoglobin RNA Precursors
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Wiebauer K
Friedrich Miescher-Institut, Basel, Switzerland.
Herrero J J
Filipowicz W
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.5.2042-2051.1988
Published
1988-05-00
Pages
2042-51
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC363383
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.8.5.2042-2051.1988 PMC Full Text View journal details
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