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PMID: 338832 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Solid-phase radioimmunoassay for detection of antibodies to myelin basic protein.

Journal of immunological methods ·Vol. 18 ·No. 3-4 ·1977-00-00 ·Pages 215-24

Randolph DH, Kibler RF, Fritz RB

Abstract

A solid-phase double-antibody radioimmunoassay was developed for the detection of anti-myelin basic protein (BP) in sera. Antigen was adsorbed to glass test tubes, reacted with rat anti-BP sera, followed by 125I-labeled rabbit anti-rat IgG. This assay was capable of detection of specific antibody at low nanogram per ml levels, was technically simple, and the results correlated well with established procedures.

MeSH Terms

Animals Antibodies Female Guinea Pigs Immune Sera/pharmacology Immunoelectrophoresis Immunosorbent Techniques Myelin Proteins/immunology Peptide Fragments/immunology Rabbits Radioimmunoassay Rats Rats, Inbred Lew Time Factors

Chemicals

Antibodies Immune Sera Myelin Proteins Peptide Fragments

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Randolph D H

Kibler R F

Fritz R B

Article Info

Journal

Journal of immunological methods

Abbr.

J Immunol Methods

ISSN

0022-1759

DOI

10.1016/0022-1759(77)90175-2

Published

1977-00-00

Pages

215-24

Language

English

Region

Netherlands

NLM ID

1305440

Subset

External Links

PubMed source DOI: 10.1016/0022-1759(77)90175-2 View journal details

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