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PMID: 3396544 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Interaction of a protein from rat liver nuclei with cruciform DNA.

The EMBO journal ·Vol. 7 ·No. 3 ·1988-03-00 ·Pages 843-9

Bianchi ME

Abstract

We constructed a synthetic cruciform DNA which closely resembles Holliday junctions, a DNA structure formed during recombination or following the transition from interstrand to intrastrand base pairing in palindromic DNA sequences. We identified and partially purified a protein from rat liver that specifically binds to this cruciform DNA molecule and does not bind to single-stranded or double-stranded DNAs of the same sequence. This protein also binds to the cruciform structure formed by a 70 bp palindromic sequence cloned in plasmid pUC18. No detectable nucleolytic activity is associated with the rat liver cruciform-binding protein, in contrast to all cruciform-recognizing proteins known so far.

MeSH Terms
Animals Cattle DNA, Single-Stranded/metabolism DNA-Binding Proteins/metabolism Liver/metabolism Molecular Conformation Nuclear Proteins/metabolism Rats
Chemicals
DNA, Single-Stranded DNA-Binding Proteins Nuclear Proteins
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Bianchi M E
EMBL, Heidelberg, FRG.
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22 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1988-03-00
Pages
843-9
Language
English
Region
England
NLM ID
8208664
PMCID
PMC454401
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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