Morphological changes and membrane potential of human granulocytes under influence of chemotactic peptide and/or echo-virus, type 9.

Klinische Wochenschrift ·Vol. 66 ·No. 10 ·1988-05-16 ·Pages 434-6

Jäger U, Gruler H, Bültmann B

Abstract

The membrane potential, Em, of human granulocytes (PMNs), was recorded using glass microelectrodes. The membrane potential Em exhibited potential fluctuations accompanied by characteristic changes of cell shape. The periodic potential fluctuations (7-s, 70-s, and 260-s periodicities) ascertained by the autocorrelation technique, suggested the existence of an internal clock. The chemoattractant f-Met-Leu-Phe (FMLP) had no influence on the periodicities, whereas the amplitudes of the fluctuations were increased by it. Treatment of PMNs with Echo 9 virus also resulted in hyperpolarization. The 70-s periodicity disappeared under virus treatment indicating a virus-induced change of the internal program and loss of chemotactic activity.

MeSH Terms

Cell Transformation, Viral/drug effects Echovirus 9/pathogenicity Enterovirus B, Human/pathogenicity Humans Membrane Potentials/drug effects N-Formylmethionine Leucyl-Phenylalanine/pharmacology Neutrophils/drug effects

Chemicals

N-Formylmethionine Leucyl-Phenylalanine

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Jäger U

Abteilung für Allgemeine Physiologie, Universität Ulm.

Gruler H

Bültmann B

References (7)

7 references, click to expand

Echo 9 virus-induced order-disorder transition of chemotactic response of human polymorphonuclear leucocytes: phenomenology and molecular biology.
Blood Cells. 1984;10(1):79-106 PMID: 6487817
Interaction of chemotactic factors with human macrophages. Induction of transmembrane potential changes.
J Cell Biol. 1977 Oct;75(1):277-89 PMID: 410816
Selective inhibition of human neutrophil chemotaxis by ECHO-virus, type 9.
Klin Wochenschr. 1981 Jun 1;59(11):571-3 PMID: 7265813
Analysis of the directed and nondirected movement of human granulocytes: influence of temperature and ECHO 9 virus on N-formylmethionylleucylphenylalanine-induced chemokinesis and chemotaxis.
J Cell Biol. 1983 Jun;96(6):1708-16 PMID: 6853601
Analysis of cell movement.
Blood Cells. 1984;10(1):61-77 PMID: 6487816
Sampling membrane potential, membrane resistance and electrode resistance with a glass electrode impaled into a single cell.
J Neurosci Methods. 1980 Apr;2(2):191-202 PMID: 7392671
Virally induced alterations in cellular permeability: a basis of cellular and physiological damage?
Biosci Rep. 1981 Jun;1(6):431-48 PMID: 7028156

Article Info

Journal

Klinische Wochenschrift

Abbr.

Klin Wochenschr

ISSN

0023-2173

DOI

10.1007/BF01745512

Published

1988-05-16

Pages

434-6

Language

English

Region

Germany

NLM ID

2985205R

Subset

External Links

PubMed source DOI: 10.1007/BF01745512

Analysis Services

Morphological changes and membrane potential of human granulocytes under influence of chemotactic peptide and/or echo-virus, type 9.

Contact

WeChat Official Account