Influence of cultivation conditions on the production of staphylocoagulase by Staphylococcus aureus 104.

High yields of staphylocoagulase from Staphylococcus aureus 104 were obtained in a simple salts medium supplement with glycerol, casein hydrolysate and three vitamins. Conditions of oxygen-limitation (dissolved oxygen concentration less than 2%), a pH of 7.4, a temperature of 35 degrees C and a 1 in 10 inoculum of overnight culture were required for optimal yields of staphylocoagulase.