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PMID: 3405213 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Upstream regulatory region for inducible expression of the chicken skeletal myosin alkali light-chain gene.

Molecular and cellular biology ·Vol. 8 ·No. 6 ·1988-06-00 ·Pages 2581-8

Shirakata M, Nabeshima Y, Konishi K, Fujii-Kuriyama Y

Abstract

The expression of the fast type of myosin alkali light chain 1 is induced during the differentiation of muscle cells. To study the mechanism of its gene regulation, we joined the sequence of the 5'-flanking and upstream region of the chicken myosin alkali light-chain gene to the structural gene for chloramphenicol acetyltransferase (CAT). The fusion gene was introduced either into quail myoblasts transformed by a temperature-sensitive mutant of Rous sarcoma virus (tsNY68) or into chicken myoblasts, and the transiently expressed CAT activity was assayed after the differentiation of the myoblasts. From the experiments with the external and internal deletion mutants of the fusion gene, the cis-acting regulatory region responsible for the enhanced expression of the CAT activity in response to the cell differentiation was found to be localized at 2 kilobases upstream of the transcription initiation site. This region of 160 nucleotides contained two pairs of short sequences worthy of note, a direct repeat of 12 nucleotides, and an inverted repeat of 8 nucleotides. The nucleotide sequences of the 5'-flanking sequence up to nucleotide -3381 were determined and compared with those of the upstream activating elements of actin genes.

MeSH Terms
Animals Base Sequence Cell Line, Transformed Cells, Cultured Chickens Gene Expression Regulation Molecular Sequence Data Mutation Myosins/genetics Regulatory Sequences, Nucleic Acid Transcription, Genetic Transfection
Chemicals
Myosins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Shirakata M
Department of Biochemistry, Cancer Institute, Japanese Foundation for Cancer Research, Tokyo.
Nabeshima Y
Konishi K
Fujii-Kuriyama Y
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.6.2581-2588.1988
Published
1988-06-00
Pages
2581-8
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC363459
Subset
IM
Databases
GENBANK
J03397
External Links
PubMed source DOI: 10.1128/mcb.8.6.2581-2588.1988 PMC Full Text View journal details
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